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Determination of plasma lysophosphatidylethanolamines (lyso-PE) by LC-MS/MS revealed a possible relation between obesity and lyso-PE in Japanese preadolescent children: The Hokkaido study.
Inoue, Nao; Gowda, Siddabasave Gowda B; Gowda, Divyavani; Sakurai, Toshihiro; Ikeda-Araki, Atsuko; Bamai, Yu Ait; Ketema, Rahel Mesfin; Kishi, Reiko; Chiba, Hitoshi; Hui, Shu-Ping.
Afiliación
  • Inoue N; Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
  • Gowda SGB; Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
  • Gowda D; Graduate School of Global Food Resources, Hokkaido University, Sapporo, Japan.
  • Sakurai T; Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
  • Ikeda-Araki A; Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
  • Bamai YA; Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
  • Ketema RM; Center for Environmental and Health Sciences, Hokkaido University, Sapporo, Japan.
  • Kishi R; Center for Environmental and Health Sciences, Hokkaido University, Sapporo, Japan.
  • Chiba H; Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
  • Hui SP; Center for Environmental and Health Sciences, Hokkaido University, Sapporo, Japan.
Ann Clin Biochem ; : 45632241280352, 2024 Sep 03.
Article en En | MEDLINE | ID: mdl-39167494
ABSTRACT

BACKGROUND:

Lysophosphatidylethanolamines (lyso-PEs) are the partial hydrolysis products of phosphatidylethanolamine. Although lyso-PEs are important biomarkers in various diseases, their determination is limited by the lack of simple and efficient quantification methods. This study aims to develop an improved quantitative method for the determination of lyso-PEs and its application to an epidemiological study.

METHODS:

Single reaction monitoring channels by collision-induced dissociation for seven lyso-PEs were established using liquid chromatography-tandem mass spectrometry. Plasma lyso-PEs were extracted with a single-phase method using an isotopically labelled internal standard for quantification. The proposed method was adopted to define lyso-PEs in plasma samples of children aged 9-12 years living in Sapporo, Japan.

RESULTS:

The limit of detection and limit of quantification for each lyso-PE ranged between 0.001-0.015 and 0.002-0.031 pmol/µL, respectively. Recoveries were found to be > 91% for all the species. The analysis results of children's plasma showed that the total lyso-PE concentrations in boys (n = 181) and girls (n = 161) were 11.53 and 11.00 pmol/µL (median), respectively. Participants were further classified by the percentage of overweight and subgrouped as underweight (n = 12), normal range (n = 292), or overweight (n = 38). Interestingly, the reduction of lyso-PE 160 and increased lyso-PE 226 were observed in overweight children compared with normal range (Fold change 0.909 and 1.174, respectively).

CONCLUSIONS:

This study successfully established a simple quantitative method to determine lyso-PE concentrations. Furthermore, our method revealed the possible relation between plasma lyso-PEs and overweight status.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Ann Clin Biochem Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Ann Clin Biochem Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido