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A simplified protocol for deriving sterile, infectious murine Heligmosomoides polygyrus bakeri larvae.
Blackwell, Karlin H; Walk, Heather M G; Peters, L Robert; Gunlikson, Emily M; Bright, Jack C; Kominsky, Douglas J; Walk, Seth T.
Afiliación
  • Blackwell KH; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA. Electronic address: karlin.havlak@student.montana.edu.
  • Walk HMG; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA.
  • Peters LR; Department of Biomedical Sciences, Western Michigan University Homer Stryker M.D., 1000 Oakland Dr., Kalamazoo, MI 49008, USA.
  • Gunlikson EM; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA.
  • Bright JC; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA.
  • Kominsky DJ; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA. Electronic address: douglas.kominsky@montana.edu.
  • Walk ST; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA. Electronic address: seth.walk@montana.edu.
STAR Protoc ; 5(3): 103144, 2024 Sep 20.
Article en En | MEDLINE | ID: mdl-39167493
ABSTRACT
Gastrointestinal helminth infection occurs within a diverse microbiome, complicating the interpretation of whether effects are caused by the parasite versus the microbial community. Here, we present a protocol for deriving sterile larvae of the murine helminth, Heligmosomoides polygyrus bakeri (H. polygyrus), providing experimental control of the microbiome. We describe steps for sterilizing with a bleach solution and developing into infectious larvae using E. coli. We then detail procedures for removing bacterial contaminants before harvesting to ensure the generation of germ-free larvae.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nematospiroides dubius / Larva Límite: Animals Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nematospiroides dubius / Larva Límite: Animals Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos