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Impact of a worker bee thoracic ganglion RIC-3 variant on the actions of acetylcholine and neonicotinoids on nicotinic receptors in Apis mellifera.
Takebayashi, Mayuka; Mori, Sumito; Ito, Ryo; Takayama, Koichi; Ojima, Hisanori; Takeuchi, Miyu; Takahashi, Hiyori; Yamamoto, Niina; Egawa, Runa; Kimura, Yuki; Ihara, Makoto; Sasaki, Ken; Sattelle, David B; Matsuda, Kazuhiko.
Afiliación
  • Takebayashi M; Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, Japan.
  • Mori S; Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, Japan.
  • Ito R; Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, Japan.
  • Takayama K; Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, Japan.
  • Ojima H; Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, Japan.
  • Takeuchi M; Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, Japan.
  • Takahashi H; Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, Japan.
  • Yamamoto N; Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, Japan.
  • Egawa R; Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, Japan.
  • Kimura Y; Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, Japan.
  • Ihara M; Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, Japan.
  • Sasaki K; Graduate School of Agriculture, Tamagawa University, Tokyo, Japan.
  • Sattelle DB; Centre for Respiratory Biology, UCL Respiratory, Division of Medicine, University College London, London, UK.
  • Matsuda K; Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, Japan.
Pest Manag Sci ; 2024 Aug 21.
Article en En | MEDLINE | ID: mdl-39167025
ABSTRACT
A transmembrane thioredoxin (TMX3) enables the functional expression of insect nicotinic acetylcholine receptors (nAChRs) in Xenopus laevis oocytes, while co-factors RIC-3 and UNC-50 regulate the receptor expression level. RIC-3 (resistant to inhibitors of cholinesterase 3) has been shown to diversify by its differential mRNA splicing patterns. How such diversity influences neonicotinoid sensitivity of nAChRs of beneficial insect species remains poorly understood. We have identified a RIC-3 variant expressed most abundantly in the thoracic ganglia of honeybee (Apis mellifera) workers and investigated its effects on the functional expression and pharmacology of Amα1/Amα8/Amß1 and Amα1/Amα2/Amα8/Amß1 nAChRs expressed in X. laevis oocytes. The AmRIC-3 enhanced the response amplitude to the acetylcholine (ACh) of these A. mellifera nAChRs when its cRNA was injected into oocytes at low concentrations but suppressed the ACh response amplitude at high concentrations. Co-expression of the AmRIC-3 had a minimal impact on the affinity of ACh, but changed the efficacy of imidacloprid and clothianidin, suggesting that the presence and the level of RIC-3 expression can affect the nAChR responses to ACh and neonicotinoids, depending on nAChR subunit composition in honeybees. © 2024 Society of Chemical Industry.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Pest Manag Sci Asunto de la revista: TOXICOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Pest Manag Sci Asunto de la revista: TOXICOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido