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Dysregulation of Aquaporin-3 and Glyceryl Glucoside Restoring Action in Hidradenitis Suppurativa in Vitro Models.
Del Vecchio, Cecilia; Gratton, Rossella; Nait-Meddour, Cécile; Nardacchione, Elena Maria; Moura, Ronald; Sommella, Eduardo; Moltrasio, Chiara; Marzano, Angelo Valerio; Ura, Blendi; Mentino, Donatella; Boniotto, Michele; d'Adamo, Adamo Pio; Calamita, Giuseppe; Crovella, Sergio; Tricarico, Paola Maura.
Afiliación
  • Del Vecchio C; Department of Medical Surgical and Health Sciences, University of Trieste, Trieste, Italy, ceciliadelvecchio2@gmail.com.
  • Gratton R; Department of Neuroscience, Scuola Internazionale Superiore di Studi Avanzati (SISSA), Trieste, Italy.
  • Nait-Meddour C; INSERM, IMRB, Translational Neuropsychiatry, University Paris Est Créteil, Créteil, France.
  • Nardacchione EM; Department of Medical Surgical and Health Sciences, University of Trieste, Trieste, Italy.
  • Moura R; Department of Advanced Diagnostics, Institute for Maternal and Child Health - IRCCS Burlo Garofolo, Trieste, Italy.
  • Sommella E; Department of Pharmacy, University of Salerno, Salerno, Italy.
  • Moltrasio C; Dermatology Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.
  • Marzano AV; Dermatology Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.
  • Ura B; Department of Pathophysiology and Transplantation, Università degli Studi di Milano, Milan, Italy.
  • Mentino D; Maternal-Neonatal Department, Institute for Maternal and Child Health - IRCCS Burlo Garofolo, Trieste, Italy.
  • Boniotto M; Department of Biosciences, Biotechnologies and Environment, University of Bari Aldo Moro, Bari, Italy.
  • d'Adamo AP; INSERM, IMRB, Translational Neuropsychiatry, University Paris Est Créteil, Créteil, France.
  • Calamita G; Department of Medical Surgical and Health Sciences, University of Trieste, Trieste, Italy.
  • Crovella S; Department of Advanced Diagnostics, Institute for Maternal and Child Health - IRCCS Burlo Garofolo, Trieste, Italy.
  • Tricarico PM; Department of Biosciences, Biotechnologies and Environment, University of Bari Aldo Moro, Bari, Italy.
Cell Physiol Biochem ; 58(4): 404-417, 2024 08 20.
Article en En | MEDLINE | ID: mdl-39166549
ABSTRACT
BACKGROUND/

AIMS:

Aquaporin-3 (AQP3) is an aquaglyceroporin and peroxiporin that plays a crucial role in skin barrier homeostasis. Dysregulated AQP3 expression has been observed in different inflammatory skin conditions. Hidradenitis Suppurativa (HS) is an autoinflammatory keratinization disease that typically appears between 10 and 21 years of age, characterized by alteration of skin barrier homeostasis.

METHODS:

To evaluate in vitro the role of AQP3 in the development of HS, we performed real-time PCR and Western blot to analyze gene and protein levels in human keratinocyte cell lines knock-out (KO) for NCSTN and PSENEN genes, simulating genetic-associated HS. Additionally, we investigated the impact of Glyceryl Glucoside (GG) on biological processes by performing MTT, scratch, proliferation assays and proteome studies.

RESULTS:

We detected a significant decrease of the levels of AQP3 gene and protein in KO cell lines. GG effectively elevated the levels of mRNA and protein, significantly decreased the hyperproliferation rate, and enhanced cell migration in our in vitro model of genetic Hidradenitis Suppurativa. Pathway enrichment analysis further confirmed GG's role in the migration and proliferation pathways of keratinocytes.

CONCLUSION:

Our results suggest that AQP3 may act as a new novel actor in HS etio-pathogenesis, and GG could be further explored as potential treatment option for managing HS in patients.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Queratinocitos / Movimiento Celular / Hidradenitis Supurativa / Proliferación Celular / Acuaporina 3 / Glucósidos Límite: Humans Idioma: En Revista: Cell Physiol Biochem Asunto de la revista: BIOQUIMICA / FARMACOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Queratinocitos / Movimiento Celular / Hidradenitis Supurativa / Proliferación Celular / Acuaporina 3 / Glucósidos Límite: Humans Idioma: En Revista: Cell Physiol Biochem Asunto de la revista: BIOQUIMICA / FARMACOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Alemania