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Induction of seizures and initiation of epileptogenesis by pilocarpine in zebrafish larvae.
Gawel, Kinga; Hulas-Stasiak, Monika; Marszalek-Grabska, Marta; Grenda, Anna; Siekierska, Aleksandra; Kosheva, Nataliia; van der Ent, Wietske; Esguerra, Camila V; Krawczyk, Pawel; Turski, Waldemar A.
Afiliación
  • Gawel K; Department of Experimental and Clinical Pharmacology, Medical University of Lublin, Lublin, Poland.
  • Hulas-Stasiak M; Department of Functional Anatomy and Cytobiology, Maria Curie-Sklodowska University, Lublin, Poland.
  • Marszalek-Grabska M; Department of Experimental and Clinical Pharmacology, Medical University of Lublin, Lublin, Poland.
  • Grenda A; Department of Pneumology, Oncology and Allergology, Medical University of Lublin, Lublin, Poland.
  • Siekierska A; VirusBank Platform, Department of Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium.
  • Kosheva N; Department of Experimental and Clinical Pharmacology, Medical University of Lublin, Lublin, Poland.
  • van der Ent W; Chemical Neuroscience Group, Centre for Molecular Medicine Norway, University of Oslo, Forskningsparken, Oslo, Norway.
  • Esguerra CV; Chemical Neuroscience Group, Centre for Molecular Medicine Norway, University of Oslo, Forskningsparken, Oslo, Norway.
  • Krawczyk P; Department of Pneumology, Oncology and Allergology, Medical University of Lublin, Lublin, Poland.
  • Turski WA; Department of Experimental and Clinical Pharmacology, Medical University of Lublin, Lublin, Poland.
Front Mol Neurosci ; 17: 1418606, 2024.
Article en En | MEDLINE | ID: mdl-39165716
ABSTRACT

Objective:

Preclinical models of seizures and epilepsy in rodents contributed substantially to the discovery of currently available antiseizure medications. These were also broadly used for investigation of processes of epileptogenesis. Nevertheless, rodent models pose some limitations, thus, new models using alternative species are in high demand. The aim of this study was to describe a new model of seizures/epilepsy induced by the cholinomimetic agent, pilocarpine (PILO), in larval zebrafish.

Methods:

Local field potential (LFP) recordings were conducted to analyze electroencephalographic discharges and correlate it with larval behavior. Hematoxylin and eosin (H&E) staining, as well as TUNEL staining were performed to analyze morphology and apoptosis, respectively. Real-time quantitative polymerase chain reaction (qRT-PCR) was undertaken for gene expression analysis.

Results:

Acute exposure to PILO, in a concentration-dependent manner, induces electroencephalographic discharges in larval zebrafish, which behaviorally manifest as decreased locomotion and moving time, but enhanced movement velocity. The PILO-induced seizure-like activity is behaviorally distinct from this induced by the application of chemoconvulsant pentylenetetrazole (PTZ). Zebrafish larvae previously exposed to PILO (2 h), after a washing out period, exhibit spontaneous, unprovoked discharges and apoptotic changes in their brains.

Significance:

Here, we comprehensively investigated a new model of PILO-induced seizures/epilepsy in larval zebrafish. We propose that this model may be used to study epileptogenesis and for antiseizure drug screening purposes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Mol Neurosci Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Mol Neurosci Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Suiza