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Development and evaluation of high-resolution melting assays for direct and simultaneous pathogen identification in bloodstream infections in pediatric oncology patients.
Lengert, Andre van Helvoort; Tassinari, Tiago Alexandre; Lourenço, Aline Taisa de Oliveira; Peronni, Kamila Chagas; Castro, Paulo de Tarso Oliveira E; Pereira, Jaqueline Estetele Massuco; Lopes, Luiz Fernando; Melendez, Matias Eliseo.
Afiliación
  • Lengert AVH; Molecular Oncology Research Center, Barretos Cancer Hospital, 1331, Antenor Duarte Vilela St, Barretos, SP, 14784-400, Brazil. Electronic address: ahlengert@gmail.com.
  • Tassinari TA; Molecular Oncology Research Center, Barretos Cancer Hospital, 1331, Antenor Duarte Vilela St, Barretos, SP, 14784-400, Brazil.
  • Lourenço ATO; Molecular Oncology Research Center, Barretos Cancer Hospital, 1331, Antenor Duarte Vilela St, Barretos, SP, 14784-400, Brazil.
  • Peronni KC; National Institute of Science and Technology in Stem Cell and Cell Therapy, Center for Cell-based Therapy-CEPID/FAPESP, Ribeirao Preto, SP, 14051-140, Brazil.
  • Castro PTOE; Barretos Cancer Hospital, 1331, Antenor Duarte Vilela St, Barretos, SP, 14784-400, Brazil.
  • Pereira JEM; Microbiology Department, Barretos Cancer Hospital, 1331, Antenor Duarte Vilela St, Barretos, SP, 14784-400, Brazil.
  • Lopes LF; Barretos Children's and Young Adults Cancer Hospital, 3025, Joao Baroni St, Barretos, SP, 14784-390, Brazil.
  • Melendez ME; Molecular Oncology Research Center, Barretos Cancer Hospital, 1331, Antenor Duarte Vilela St, Barretos, SP, 14784-400, Brazil; National Cancer Institute, 37, André Cavalcanti St, Rio de Janeiro, RJ, 20231-050, Brazil. Electronic address: matias.melendez@inca.gov.br.
Diagn Microbiol Infect Dis ; 110(3): 116426, 2024 Nov.
Article en En | MEDLINE | ID: mdl-39163789
ABSTRACT
Blood culture, the gold-standard method for identifying pathogens in bloodstream infections, is time-consuming and demonstrates low sensitivity. These drawbacks are related to high mortality, especially among pediatric oncology patients presenting febrile neutropenia episodes. Here we describe two novel High-Resolution Melting assays designed for pathogen detection in bloodstream infections. The assays were initially evaluated using five sepsis-associated pathogens. Both assays demonstrated 100 % specificity, detected as low as 100 fg of bacterial DNA, and exhibited reproducibility. Clinical isolates from blood cultures were 100 % identified by both assays. Moreover, blind and direct identification of blood samples from pediatric cancer patients demonstrated sensitivities of 61.5 % and 69.2 % for "Primer Set 1" and "Primer Set 2", respectively. Our study highlights the potential of HRM-based assays as a rapid and efficient diagnostic approach for sepsis-related microorganisms. Further advancements could enhance their clinical utility for better management of febrile neutropenia episodes, especially in pediatric oncology patients.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sensibilidad y Especificidad / Neoplasias Límite: Child / Child, preschool / Humans / Infant Idioma: En Revista: Diagn Microbiol Infect Dis Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sensibilidad y Especificidad / Neoplasias Límite: Child / Child, preschool / Humans / Infant Idioma: En Revista: Diagn Microbiol Infect Dis Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos