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Functionally improved mesenchymal stem cells via nanosecond pulsed electric fields for better treatment of osteoarthritis.
Lin, Jianjing; Li, Kejia; Yang, Zhen; Cao, Fuyang; Gao, Liang; Ning, Tong; Xing, Dan; Zeng, Hui; Liu, Qiang; Ge, Zigang; Lin, Jianhao.
Afiliación
  • Lin J; Arthritis Clinical and Research Center, Peking University People's Hospital, No.11 Xizhimen South Street, Beijing, 100044, China.
  • Li K; Arthritis Institute, Peking University, Beijing, 100044, China.
  • Yang Z; Department of Sports Medicine and Rehabilitation, Peking University Shenzhen Hospital, Shenzhen, 518036, China.
  • Cao F; Department of Biomedical Engineering, Institute of Future Technology, Peking University, Beijing, 100871, China.
  • Gao L; Institute for Tissue Engineering and Regenerative Medicine, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong Special Administrative Region of China.
  • Ning T; Arthritis Clinical and Research Center, Peking University People's Hospital, No.11 Xizhimen South Street, Beijing, 100044, China.
  • Xing D; Arthritis Institute, Peking University, Beijing, 100044, China.
  • Zeng H; Department of Orthopedics, Second Hospital of Shanxi Medical University, Taiyuan, 030001, China.
  • Liu Q; Department of Orthopaedics, The First Affiliated Hospital of Anhui Medical University, Hefei, 230041, China.
  • Ge Z; Institute of Medical Science, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250033, China.
  • Lin J; Arthritis Clinical and Research Center, Peking University People's Hospital, No.11 Xizhimen South Street, Beijing, 100044, China.
J Orthop Translat ; 47: 235-248, 2024 Jul.
Article en En | MEDLINE | ID: mdl-39161657
ABSTRACT

Background:

Numerous approaches have been utilized to optimize mesenchymal stem cells (MSCs) performance in treating osteoarthritis (OA), however, the constrained diminished activity and chondrogenic differentiation capacity impede their therapeutic efficacy. Previous investigations have successfully shown that pretreatment with nanosecond pulsed electric fields (nsPEFs) significantly enhances the chondrogenic differentiation of MSCs. Therefore, this study aims to explore nsPEFs as a strategy to improve OA therapy by enhancing MSCs' activity and chondrogenic differentiation and also investigate its potential mechanism.

Methods:

In this study, a million MSCs were carefully suspended within a 0.4-cm gap cuvette and subjected to five pulses of nsPEFs (100 ns at 10 kV/cm, 1 Hz), with a 1-s interval between each pulse. A control group of MSCs was maintained without nsPEFs treatment for comparative analysis. nsPEFs were applied to regulate the MSCs performance and hinder OA progresses. In order to further explore the corresponding mechanism, we examined the changes of MSCs transcriptome after nsPEF pretreatment. Finally, we studied the properties of extracellular vesicles (EVs) secreted by MSCs affected by nsPEF and the therapeutic effect on OA.

Results:

We found that nsPEFs pretreatment promoted MSCs migration and viability, particularly enhancing their viability temporarily in vivo, which is also confirmed by mRNA sequencing analysis. It also significantly inhibited the development of OA-like chondrocytes in vitro and prevented OA progression in rat models. Additionally, we discovered that nsPEFs pretreatment reprogrammed MSC performance by enhancing EVs production (5.77 ± 0.92 folds), and consequently optimizing their therapeutic potential.

Conclusions:

In conclusion, nsPEFs pretreatment provides a simple and effective strategy for improving the MSCs performance and the therapeutic effects of MSCs for OA. EVs-nsPEFs may serve as a potent therapeutic material for OA and hold promise for future clinical applications. The translational potential of this article This study indicates that MSCs pretreated by nsPEFs greatly inhibited the development of OA. nsPEFs pretreatment will be a promising and effective method to optimize the therapeutic effect of MSCs in the future.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Orthop Translat Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Singapur
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Orthop Translat Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Singapur