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Circular RNA LDLRAD3 promotes gastric cancer progression by upregulating COL4A5 through sponging miR-h37.
Li, Chenghui; Xing, Xiao; Huang, Sinian; Zhu, Ting; Yan, Bin.
Afiliación
  • Li C; Department of General Surgery, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai, China.
  • Xing X; Department of General Surgery, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai, China.
  • Huang S; Department of Pathology Surgery, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai, China.
  • Zhu T; Department of Pathology Surgery, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai, China.
  • Yan B; Department of General Surgery, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai, China.
J Chin Med Assoc ; 87(11): 1018-1028, 2024 Nov 01.
Article en En | MEDLINE | ID: mdl-39161132
ABSTRACT

BACKGROUND:

Circular RNAs play an important role in the development of gastric cancer (GC). circ-low-density lipoprotein receptor class A domain containing 3 (LDLRAD3) has been confirmed to be related to GC progression. miR-137 is also a suppressor in GC. However, the impact of the interaction between circ-LDLRAD3 and miR-137 on the progression of GC remains unclear at present.

METHODS:

The study identified expression level differences of circ-LDLRAD3, miR-137, and COL4A5 in GC pathological specimens compared to normal tissue samples. Furthermore, through in vitro experiments, including flow cytometry, cell counting kit-8 (CCK-8) assays, wound healing, Western blotting, and colony formation assays, we further explored the molecular regulatory mechanisms by which these factors promote the progression of GC.

RESULTS:

In this study, circ-LDLRAD3 was confirmed to have higher expression, and miR-137 had lower expression in GC tissues and cell lines. circ-LDLRAD3 knockdown and miR-137 overexpression promoted apoptosis and inhibited proliferation, migration, and invasion in GC cell lines. Further experiments validated that COL4A5 had a positive relationship with GC and that circ-LDLRAD3 promoted the expression of COL4A5. circ-LDLRAD3 could be sponged and inhibited by miR-137 in GC cells. As a result, the promotional effect of circ-LDLRAD3 on COL4A5 was counteracted by miR-137.

CONCLUSION:

Our study showed that the knockdown of circ-LDLRAD3 suppressed the development of GC by regulating the miR-137/COL4A5 axis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Gástricas / Regulación hacia Arriba / Progresión de la Enfermedad / MicroARNs / ARN Circular Límite: Humans Idioma: En Revista: J Chin Med Assoc Asunto de la revista: MEDICINA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Gástricas / Regulación hacia Arriba / Progresión de la Enfermedad / MicroARNs / ARN Circular Límite: Humans Idioma: En Revista: J Chin Med Assoc Asunto de la revista: MEDICINA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos