Complexity in a Simple Self-Assembling System: Lecithin-Water-Ethanol Mixtures Exhibit a Re-Entrant Phase Transition and a Vesicle-Micelle Transition (VMT) on Heating.
Langmuir
; 2024 Aug 19.
Article
en En
| MEDLINE
| ID: mdl-39158103
ABSTRACT
We report surprising results for the self-assembly of lecithin (a common phospholipid) in water-ethanol mixtures. Lecithin forms vesicles (â¼100 nm diameter) in water. These vesicles are transformed into small micelles (â¼5 nm diameter) by a variety of destabilizing agents such as single-tailed surfactants and alcohols. In a surfactant-induced vesicle-micelle transition (VMT), vesicles steadily convert to micelles upon adding the surfactantâthereby, the turbidity of the solution drops monotonically. Instead, when an alcohol like ethanol is added to lecithin vesicles, we find a new, distinctive pattern in phase behavior as the ethanol fraction feth in water is increased. The turbidity first decreases (from feth = 0 to 37%), then rises sharply (feth = 37 to 50%), and then eventually decreases again (feth > 55%). Concomitant with the turbidity rise, the vesicles separate into two phases around feth = 50% before a single phase reappears at higher fethâin other words, there is a "re-entrant" phase transition from 1-phase to 2-phase and back to 1-phase with increasing feth. Vesicles near the phase boundary (â¼feth = 45%) also show a VMT upon heating. Similar patterns are seen with other alcohols such as methanol and propanol. We ascribe these complex trends to the dual role played by alcohols (a) first, alcohols reduce the propensity for flat lipid bilayers to bend and form closed spherical vesicles; and (b) second, alcohols diminish the tendency of lipids to self-assemble in the solvent mixture. At low alcohol fractions, (a) dominates, causing the initially unilamellar vesicles to grow into multilamellar vesicles (MLVs), which eventually phase-separate. Thereafter, (b) dominates, and the vesicles convert into micelles. Support for our hypothesis comes from scattering (SANS) and microscopy (cryo-TEM). Thus, we have uncovered a general paradigm for lipid self-assembly in solvent mixtures, and this may even have physiological relevance.
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Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Langmuir
Asunto de la revista:
QUIMICA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos