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Engineering of Recombinant Human Papillomavirus 16 L1 Protein for Incorporation with para-Azido-L-Phenylalanine.
Kim, Jinhyeon; Jeong, Ki Jun; Kim, Geun-Joong; Choi, Jong-Il.
Afiliación
  • Kim J; Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 61186, Republic of Korea.
  • Jeong KJ; Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 34141, Republic of Korea.
  • Kim GJ; Department of Biological Sciences and Research Center of Ecomimetics, Chonnam National University, Gwangju 61186, Republic of Korea.
  • Choi JI; Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 61186, Republic of Korea.
J Microbiol Biotechnol ; 34(9): 1-7, 2024 Aug 09.
Article en En | MEDLINE | ID: mdl-39155395
ABSTRACT
Human papillomavirus (HPV) L1 capsid protein were produced in several host systems, but few studies have focused on enhancing the properties of the L1 protein. In this study, we aimed to produce recombinant Human papillomavirus (HPV) L1 capsid protein containing para-azido-L-phenylalanine (pAzF) in Escherichia coli. First, we expressed the maltose-binding protein (MBP)- fused HPV16 L1, and 5 residues in HPV16 L1 protein were selected by the in silico modeling for amber codon substitution. Among the variants of the five locations, we identified a candidate that exhibited significant differences in expression with and without pAzF via genetic code expansion (GCE). The expressed recombinant MBP-HPV16L1 protein was confirmed for incorporation of pAzF and the formation of VLPs was tested in vitro.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Microbiol Biotechnol Año: 2024 Tipo del documento: Article Pais de publicación:
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Microbiol Biotechnol Año: 2024 Tipo del documento: Article Pais de publicación: