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Antimicrobial Silk Fibroin Methacrylated Scaffolds for Regenerative Endodontics.
Narayanam, Ramyasaketha; Cardoso, Lais M; Dos Reis-Prado, Alexandre H; de Carvalho, Ana Beatriz G; Anselmi, Caroline; Mahmoud, Abdel H; Fenno, J Christopher; Dal-Fabbro, Renan; Bottino, Marco C.
Afiliación
  • Narayanam R; Department of Cariology, Restorative Sciences and Endodontics, University of Michigan-School of Dentistry, Ann Arbor, Michigan.
  • Cardoso LM; Department of Cariology, Restorative Sciences and Endodontics, University of Michigan-School of Dentistry, Ann Arbor, Michigan; Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP)-Araraquara School of Dentistry, Araraquara, São Paulo, Brazil.
  • Dos Reis-Prado AH; Department of Cariology, Restorative Sciences and Endodontics, University of Michigan-School of Dentistry, Ann Arbor, Michigan; Department of Restorative Dentistry, Minas Gerais Federal University (UFMG)-School of Dentistry, Belo Horizonte, Minas Gerais, Brazil.
  • de Carvalho ABG; Department of Cariology, Restorative Sciences and Endodontics, University of Michigan-School of Dentistry, Ann Arbor, Michigan; Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP)-Sao Jose dos Campos School of Dentistry, São José dos Campos, São Paulo, Brazil.
  • Anselmi C; Department of Cariology, Restorative Sciences and Endodontics, University of Michigan-School of Dentistry, Ann Arbor, Michigan; Department of Morphology and Pediatric Dentistry, São Paulo State University (UNESP)-Araraquara School of Dentistry, Araraquara, São Paulo, Brazil.
  • Mahmoud AH; Department of Oral Biology and Pathology, Stony Brook University-School of Dental Medicine, Stony Brook, New York.
  • Fenno JC; Department of Biologic and Materials Sciences & Prosthodontics, University of Michigan-School of Dentistry, Ann Arbor, Michigan.
  • Dal-Fabbro R; Department of Cariology, Restorative Sciences and Endodontics, University of Michigan-School of Dentistry, Ann Arbor, Michigan.
  • Bottino MC; Department of Cariology, Restorative Sciences and Endodontics, University of Michigan-School of Dentistry, Ann Arbor, Michigan; Department of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan. Electronic address: mbottino@umich.edu.
J Endod ; 2024 Aug 21.
Article en En | MEDLINE | ID: mdl-39155022
ABSTRACT

INTRODUCTION:

Recognizing the necessity of novel disinfection strategies for improved bacterial control to ultimately favor tissue regeneration, this study developed and characterized antibiotics-laden silk fibroin methacrylated (SilkMA) scaffolds for regenerative endodontics.

METHODS:

SilkMA-based solutions (10% w/v) containing Clindamycin (CLI) or Tinidazole (TIN) (0 - control; 5, 10, or 15% w/w) or the combination of both drugs (BiMix CLI/TIN 10%) were electrospun and photocrosslinked. Morphology and composition were assessed using scanning electron microscopy and Fourier-transform infrared spectroscopy. Additionally, swelling and degradation profiles were also determined. Cytotoxicity was evaluated in stem cells from apical papilla. Antibacterial efficacy was tested using direct and indirect contact assays against Aggregatibacter actinomycetemcomitans/Aa, Actinomyces naeslundii/An, Enterococcus faecalis/Ef, and Fusobacterium nucleatum/Fn. E. faecalis biofilm inhibition on dentin discs was specifically evaluated for BiMix-laden scaffolds. Data were statistically analyzed with a significance level of 5%.

RESULTS:

Scanning electron microscopy revealed that all scaffolds had similar characteristics, including fiber morphology and bead absence. Fourier-transform infrared spectroscopy showed the incorporation of CLI and TIN into the fibers and in BiMix scaffolds. Antibiotic-laden scaffolds exhibited lower swelling capacity than the control and were degraded entirely after 45 days. Scaffolds laden with CLI, TIN, or BiMix throughout all time points did not reduce stem cells from apical papilla's viability. CLI-laden scaffolds inhibited the growth of Aa, An, and Ef, while TIN-laden scaffolds inhibited Fn growth. BiMix-laden scaffolds significantly inhibited Aa, An, Ef, and Fn in direct contact, and their aliquots inhibited An and Fn through indirect contact, with additional biofilm inhibition against Ef.

CONCLUSIONS:

BiMix-laden SilkMA scaffolds are cytocompatible and exhibit antimicrobial effects against endodontic pathogens, indicating their therapeutic potential as a drug delivery system for regenerative endodontics.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Endod Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Endod Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos