Heterologous expression and purification of the phage lysin-like bacteriocin LysL from Lactococcus lactis LAC460.
FEMS Microbiol Lett
; 3712024 Jan 09.
Article
en En
| MEDLINE
| ID: mdl-39153967
ABSTRACT
The wild-type Lactococcus lactis strain LAC460 produces two bacteriocin-like phage lysins, LysL and LysP. This study aimed to produce and secrete LysL in various heterologous hosts and an in vitro cell-free expression system for further functional studies. Initially, the lysL gene from L. lactis LAC460 was cloned into Lactococcus cremoris NZ9000 and L. lactis N8 strains, with and without the usp45 signal sequence (SSusp45), under a nisin-inducible promoter. Active LysL was primarily produced intracellularly in recombinant L. lactis N8, with some secretion into the supernatant. Recombinant L. cremoris NZ9000 lysed upon nisin induction, indicating successful lysL expression. However, fusion with Usp45 signal peptide (SPUsp45-LysL) weakened LysL activity, likely due to incomplete signal peptide cleavage during secretion. Active LysL was also produced in vitro, and analysed in SDS-PAGE, giving a 42-kDa band. However, the yield of LysL protein was still low when produced from recombinant lactococci or by in vitro expression system. Therefore, His-tagged LysL was produced in Escherichia coli BL21(DE3). Western blot confirmed the intracellular production of about 44-kDa His-tagged LysL in E. coli. His-tagged active LysL was then purified by Ni-NTA affinity chromatography yielding sufficient 4.34 mg of protein to be used in future functional studies.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacteriocinas
/
Lactococcus lactis
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
2024
Tipo del documento:
Article
País de afiliación:
Finlandia
Pais de publicación:
Reino Unido