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Metal Oxide Nanoparticles Exhibit Anti-Acanthamoeba castellanii Properties by Inducing Necrotic Cell Death.
Ahmed, Usman; Gew, Lai Ti; Siddiqui, Ruqaiyyah; Khan, Naveed Ahmed; Alharbi, Ahmad M; Alhazmi, Ayman; Anwar, Ayaz.
Afiliación
  • Ahmed U; Department of Biological Sciences, School of Medical and Life Sciences, Sunway University, 47500, Subang Jaya, Selangor, Malaysia.
  • Gew LT; Department of Biological Sciences, School of Medical and Life Sciences, Sunway University, 47500, Subang Jaya, Selangor, Malaysia.
  • Siddiqui R; Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University Edinburgh, Edinburgh, EH14 4AS, UK.
  • Khan NA; Microbiota Research Center, Istinye University, 34010, Istanbul, Turkey.
  • Alharbi AM; Microbiota Research Center, Istinye University, 34010, Istanbul, Turkey. naveed5438@gmail.com.
  • Alhazmi A; Department of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, 21944, Taif, Saudi Arabia.
  • Anwar A; Department of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, 21944, Taif, Saudi Arabia.
Acta Parasitol ; 69(3): 1717-1723, 2024 Sep.
Article en En | MEDLINE | ID: mdl-39153011
ABSTRACT

PURPOSE:

The treatment of amoebic infections is often problematic, largely due to delayed diagnosis, amoebae transformation into resistant cyst form, and lack of availability of effective chemotherapeutic agents. Herein, we determined anti-Acanthamoeba castellanii properties of three metal oxide nanoparticles (TiO2, ZrO2, and Al2O3).

METHODS:

Amoebicidal assays were performed to determine whether metal oxide nanoparticles inhibit amoebae viability. Encystation assays were performed to test whether metal oxide nanoparticles inhibit cyst formation. By measuring lactate dehydrogenase release, cytotoxicity assays were performed to determine human cell damage. Hoechst 33342/PI staining was performed to determine programmed cell death (apoptosis) and necrosis in A. castellanii.

RESULTS:

TiO2-NPs significantly inhibited amoebae viability as observed through amoebicidal assays, as well as inhibited their phenotypic transformation as evident using encystation assays, and showed limited human cell damage as observed by measuring lactate dehydrogenase assays. Furthermore, TiO2-NPs altered parasite membranes and resulted in necrotic cell death as determined using double staining cell death assays with Hoechst33342/Propidium iodide (PI) observed through chromatin condensation. These findings suggest that TiO2-NPs offers a potential viable avenue in the rationale development of therapeutic interventions against Acanthamoeba infections.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acanthamoeba castellanii / Nanopartículas del Metal / Necrosis Límite: Humans Idioma: En Revista: Acta Parasitol Año: 2024 Tipo del documento: Article País de afiliación: Malasia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acanthamoeba castellanii / Nanopartículas del Metal / Necrosis Límite: Humans Idioma: En Revista: Acta Parasitol Año: 2024 Tipo del documento: Article País de afiliación: Malasia Pais de publicación: Suiza