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The genetically encoded calcium indicator GCaMP3 reveals spontaneous calcium oscillations at asexual stages of the human malaria parasite Plasmodium falciparum.
Dos Santos, Benedito M; Pecenin, Mateus F; Borges-Pereira, Lucas; Springer, Eric; Przyborski, Jude M; Martins-Jr, David C; Hashimoto, Ronaldo F; Garcia, Célia R S.
Afiliación
  • Dos Santos BM; Department of Clinical and Toxicological Analysis, School of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.
  • Pecenin MF; Department of Clinical and Toxicological Analysis, School of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.
  • Borges-Pereira L; Department of Clinical and Toxicological Analysis, School of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.
  • Springer E; Department of Biochemistry and Molecular Biology, Interdisciplinary Research Center, Justus-Liebig University, Gießen 35390, Germany.
  • Przyborski JM; Department of Biochemistry and Molecular Biology, Interdisciplinary Research Center, Justus-Liebig University, Gießen 35390, Germany.
  • Martins-Jr DC; Center of Mathematics, Computing and Cognition, Federal University of ABC, Santo André 09606-045, Brazil.
  • Hashimoto RF; Institute of Mathematics and Statistics, University of São Paulo, São Paulo 05508-000, Brazil.
  • Garcia CRS; Department of Clinical and Toxicological Analysis, School of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-000, Brazil. Electronic address: celiaregarcia@gmail.com.
Mol Biochem Parasitol ; 260: 111650, 2024 Aug 14.
Article en En | MEDLINE | ID: mdl-39151473
ABSTRACT
Most protocols used to study the dynamics of calcium (Ca2+) in the malaria parasite are based on dyes, which are invasive and do not allow discrimination between the signal from the host cell and the parasite. To avoid this pitfall, we have generated a parasite line expressing the genetically encoded calcium sensor GCaMP3. The PfGCaMP3 parasite line is an innovative tool for studying spontaneous intracellular Ca2+ oscillations without external markers. Using this parasite line, we demonstrate the occurrence of spontaneous Ca2+ oscillations in the ring, trophozoite, and schizont stages in Plasmodium falciparum. Using the Fourier transform to fluorescence intensity data extracted from different experiments, we observe cytosolic Ca2+ fluctuations. These spontaneous cytosolic Ca2+ oscillations occur in the three intraerythrocytic stages of the parasite, with most oscillations occurring in the ring and trophozoite stages. A control parasite line expressing only a GFP control did not reveal such fluctuations, demonstrating the specificity of the observations. Our results clearly show dynamic, spontaneous Ca2+ oscillations during the asexual stage in P. falciparum, independent from external stimuli.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Mol Biochem Parasitol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Mol Biochem Parasitol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos