Your browser doesn't support javascript.
loading
Sensitive electrochemical immunosensor for rapid detection of Salmonella in milk using polydopamine/CoFe-MOFs@Nafion modified gold electrode.
Yi, Zhibin; Zhang, Yu; Gao, Xiaoyu; Li, Shuang; Li, Kexin; Xiong, Chunhong; Huang, Ganhui; Zhang, Jinsheng.
Afiliación
  • Yi Z; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China.
  • Zhang Y; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China.
  • Gao X; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China.
  • Li S; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China.
  • Li K; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China.
  • Xiong C; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China.
  • Huang G; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China.
  • Zhang J; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China. Electronic address: zhangjinsheng@ncu.edu.cn.
Int J Food Microbiol ; 425: 110870, 2024 Dec 02.
Article en En | MEDLINE | ID: mdl-39151230
ABSTRACT
Food contaminated by pathogenic bacteria poses a serious threat to human health. Consequently, we used Salmonella as a model and developed an electrochemical immunosensor based on a polydopamine/CoFe-MOFs@Nafion nanocomposite for the detection of Salmonella in milk. The CoFe-MOFs exhibit good stability, large specific surface area, and high porosity. However, after modification on the electrode surface, they were prone to detachment. This issue was effectively mitigated by incorporating Nafion into the nanocomposite. A polydopamine (PDA) film was deposited onto the surface of CoFe-MOFs@Nafion through cyclic voltammetry (CV), accompanied by an investigation into the polymerization mechanism of the PDA film. PDA contains a substantial number of quinone functional groups, which can covalently bind to amino or sulfhydryl groups via Michael addition reaction or Schiff base reaction, thereby immobilizing anti-Salmonella antibodies onto the modified electrode surface. Under the optimal experimental conditions, the Salmonella concentration exhibited a good linear relationship within the range of 1.38 × 102 to 1.38 × 108 CFU mL-1, with a detection limit of 1.38 × 102 CFU mL-1. Furthermore, the constructed immunosensor demonstrated good specificity, stability, and reproducibility, offering a novel approach for the rapid detection of foodborne pathogens.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Salmonella / Polímeros de Fluorocarbono / Técnicas Biosensibles / Leche / Electrodos / Técnicas Electroquímicas / Oro / Indoles Límite: Animals Idioma: En Revista: Int J Food Microbiol Asunto de la revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Salmonella / Polímeros de Fluorocarbono / Técnicas Biosensibles / Leche / Electrodos / Técnicas Electroquímicas / Oro / Indoles Límite: Animals Idioma: En Revista: Int J Food Microbiol Asunto de la revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos