Your browser doesn't support javascript.
loading
Electron Transfer Drives the Photosensitized Polymerization of Contrast Agents by Flavoprotein Tags for Correlative Microscopy.
Lone, Mohd Sajid; Merino-Chavez, Olga D; Ricks, Nathan J; Hammond, Ming C; Noriega, Rodrigo.
Afiliación
  • Lone MS; Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, United States.
  • Merino-Chavez OD; Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, United States.
  • Ricks NJ; Henry Eyring Center for Cell and Genome Science, University of Utah, Salt Lake City, Utah 84112, United States.
  • Hammond MC; Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, United States.
  • Noriega R; Henry Eyring Center for Cell and Genome Science, University of Utah, Salt Lake City, Utah 84112, United States.
J Am Chem Soc ; 146(34): 23797-23805, 2024 Aug 28.
Article en En | MEDLINE | ID: mdl-39150381
ABSTRACT
Singlet oxygen generation has long been considered the key feature that allows genetically encoded fluorescent tags to produce polymeric contrast agents for electron microscopy. Optimization of the singlet oxygen sensitization quantum yield has not included the effects of electron-rich monomers on the sensitizer's photocycle. We report that at monomer concentrations employed for staining, quenching by electron transfer is the primary deactivation pathway for photoexcitations. A simple photochemical model including contributions from both processes reproduces the observed reaction rates and indicates that most of the product is driven by pathways that involve electron transfer with monomers─not by the sensitization of singlet oxygen. Realizing the importance of these competing reaction pathways offers a new paradigm to guide the development of genetically encodable tags and suggests opportunities to expand the materials scope and growth conditions for polymeric contrast agents (e.g., biocompatible monomers, O2 poor environments).
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Medios de Contraste / Polimerizacion Idioma: En Revista: J Am Chem Soc Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Medios de Contraste / Polimerizacion Idioma: En Revista: J Am Chem Soc Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos