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A synthetic cell-free pathway for biocatalytic upgrading of one-carbon substrates.
Landwehr, Grant M; Vogeli, Bastian; Tian, Cong; Singal, Bharti; Gupta, Anika; Lion, Rebeca; Sargent, Edward H; Karim, Ashty S; Jewett, Michael C.
Afiliación
  • Landwehr GM; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, 60208, USA.
  • Vogeli B; Center for Synthetic Biology, Northwestern University, Evanston, IL, 60208, USA.
  • Tian C; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, 60208, USA.
  • Singal B; Center for Synthetic Biology, Northwestern University, Evanston, IL, 60208, USA.
  • Gupta A; Department of Chemistry, Northwestern University, Evanston, IL, 60208, USA.
  • Lion R; Stanford SLAC CryoEM Initiative, Stanford University; Stanford, CA 94305, USA.
  • Sargent EH; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, 60208, USA.
  • Karim AS; Center for Synthetic Biology, Northwestern University, Evanston, IL, 60208, USA.
  • Jewett MC; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, 60208, USA.
bioRxiv ; 2024 Aug 08.
Article en En | MEDLINE | ID: mdl-39149402
ABSTRACT
Biotechnological processes hold tremendous potential for the efficient and sustainable conversion of one-carbon (C1) substrates into complex multi-carbon products. However, the development of robust and versatile biocatalytic systems for this purpose remains a significant challenge. In this study, we report a hybrid electrochemical-biochemical cell-free system for the conversion of C1 substrates into the universal biological building block acetyl-CoA. The synthetic reductive formate pathway (ReForm) consists of five core enzymes catalyzing non-natural reactions that were established through a cell-free enzyme engineering platform. We demonstrate that ReForm works in a plug-and-play manner to accept diverse C1 substrates including CO2 equivalents. We anticipate that ReForm will facilitate efforts to build and improve synthetic C1 utilization pathways for a formate-based bioeconomy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: BioRxiv Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: BioRxiv Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos