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[Role of reactive oxygen species/silent information regulator 1 in hyperoxia-induced bronchial epithelial cell injury]. / 活性氧簇/沉默信息调节因子1在高氧致支气管上皮细胞损伤中的作用.
Yang, Kun; Wu, Yue; Zhang, Rong; Lei, Xiao-Ping; Kang, Lan; Dong, Wen-Bin.
Afiliación
  • Yang K; Department of Neonatology, Children's Medical Center, Affiliated Hospital of Southwest Medical University/Sichuan Clinical Research Center for Birth Defects, Luzhou, Sichuan 646000, China.
  • Wu Y; Department of Neonatology, Children's Medical Center, Affiliated Hospital of Southwest Medical University/Sichuan Clinical Research Center for Birth Defects, Luzhou, Sichuan 646000, China.
  • Zhang R; Department of Neonatology, Children's Medical Center, Affiliated Hospital of Southwest Medical University/Sichuan Clinical Research Center for Birth Defects, Luzhou, Sichuan 646000, China.
  • Lei XP; Department of Neonatology, Children's Medical Center, Affiliated Hospital of Southwest Medical University/Sichuan Clinical Research Center for Birth Defects, Luzhou, Sichuan 646000, China.
  • Kang L; Department of Neonatology, Children's Medical Center, Affiliated Hospital of Southwest Medical University/Sichuan Clinical Research Center for Birth Defects, Luzhou, Sichuan 646000, China.
  • Dong WB; Department of Neonatology, Children's Medical Center, Affiliated Hospital of Southwest Medical University/Sichuan Clinical Research Center for Birth Defects, Luzhou, Sichuan 646000, China.
Zhongguo Dang Dai Er Ke Za Zhi ; 26(8): 852-860, 2024 Aug 15.
Article en Zh | MEDLINE | ID: mdl-39148391
ABSTRACT

OBJECTIVES:

To investigate the effect of reactive oxygen species (ROS)/silent information regulator 1 (SIRT1) on hyperoxia-induced mitochondrial injury in BEAS-2B cells.

METHODS:

The experiment was divided into three parts. In the first part, cells were divided into H0, H6, H12, H24, and H48 groups. In the second part, cells were divided into control group, H48 group, H48 hyperoxia+SIRT1 inhibitor group (H48+EX 527 group), and H48 hyperoxia+SIRT1 agonist group (H48+SRT1720 group). In the third part, cells were divided into control group, 48-hour hyperoxia+N-acetylcysteine group (H48+NAC group), and H48 group. The ROS kit was used to measure the level of ROS. Western blot and immunofluorescent staining were used to measure the expression levels of SIRT1 and mitochondria-related proteins. Transmission electron microscopy was used to observe the morphology of mitochondria.

RESULTS:

Compared with the H0 group, the H6, H12, H24, and H48 groups had a significantly increased fluorescence intensity of ROS (P<0.05), the H48 group had significant reductions in the expression levels of SIRT1 protein and mitochondria-related proteins (P<0.05), and the H24 and H48 groups had a significant reduction in the fluorescence intensity of mitochondria-related proteins (P<0.05). Compared with the H48 group, the H48+SRT1720 group had significant increases in the expression levels of mitochondria-related proteins and the mitochondrial aspect ratio (P<0.05), and the H48+EX 527 group had a significant reduction in the mitochondrial area (P<0.05). Compared with the H48 group, the H48+NAC group had a significantly decreased fluorescence intensity of ROS (P<0.05) and significantly increased levels of SIRT1 protein, mitochondria-related proteins, mitochondrial area, and mitochondrial aspect ratio (P<0.05).

CONCLUSIONS:

The ROS/SIRT1 axis is involved in hyperoxia-induced mitochondrial injury in BEAS-2B cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bronquios / Especies Reactivas de Oxígeno / Hiperoxia / Células Epiteliales / Sirtuina 1 Límite: Humans Idioma: Zh Revista: Zhongguo Dang Dai Er Ke Za Zhi Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: China
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bronquios / Especies Reactivas de Oxígeno / Hiperoxia / Células Epiteliales / Sirtuina 1 Límite: Humans Idioma: Zh Revista: Zhongguo Dang Dai Er Ke Za Zhi Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: China