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Survival of Twelve Pathogenic and Generic Escherichia coli Strains in Agricultural Soils as Influenced by Strain, Soil Type, Irrigation Regimen, and Soil Amendment.
Murphy, Claire M; Weller, Daniel L; Bardsley, Cameron A; Ingram, David T; Chen, Yuhuan; Oryang, David; Rideout, Steven L; Strawn, Laura K.
Afiliación
  • Murphy CM; School of Food Science, Washington State University - Irrigated Agriculture Research and Extension Center, Prosser, Washington, USA; Department of Food Science and Technology, Virginia Tech, Blacksburg, Virginia, USA.
  • Weller DL; Department of Food Science and Technology, Virginia Tech, Blacksburg, Virginia, USA.
  • Bardsley CA; Department of Food Science and Technology, Virginia Tech, Blacksburg, Virginia, USA.
  • Ingram DT; Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, Maryland, USA.
  • Chen Y; Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, Maryland, USA.
  • Oryang D; Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, Maryland, USA.
  • Rideout SL; School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, Virginia, USA.
  • Strawn LK; Department of Food Science and Technology, Virginia Tech, Blacksburg, Virginia, USA. Electronic address: lstrawn@vt.edu.
J Food Prot ; 87(10): 100343, 2024 Aug 13.
Article en En | MEDLINE | ID: mdl-39147099
ABSTRACT
Biological soil amendments of animal origin (BSAAO) play an important role in agriculture but can introduce pathogens into soils. Pathogen survival in soil is widely studied, but data are needed on the impacts of strain variability and field management practices. This study monitored the population of 12 Escherichia coli strains (generic, O157, and non-O157) in soils while evaluating the interactions of soil type, irrigation regimen, and soil amendment in three independent, greenhouse-based, randomized complete block design trials. Each E. coli strain (4-5 log10 CFU/g) was homogenized in bovine manure amended or nonamended sandy-loam or clay-loam soil. E. coli was enumerated in 25 g samples on 0, 0.167 (4 h), 1, 2, 4, 7, 10, 14, 21, 28, 56, 84, 112, 168, 210, 252, and 336 days postinoculation (dpi). Regression analyses were developed to understand the impact of strain, soil type, irrigation regimen, and soil amendment on inactivation rates. E. coli survived for 112 to 336 dpi depending on the treatment combination. Pathogenic and generic E. coli survived 46 days [95% Confidence interval (CI) = 20.85, 64.72; p = 0.001] longer in soils irrigated weekly compared to daily and 146 days (CI = 114.50, 184.50; p < 0.001) longer in amended soils compared to unamended soils. Pathogenic E. coli strains were nondetectable 69 days (CI = 39.58, 98.66, p = 0.015) earlier than generic E. coli strains. E. coli inactivation rates demonstrated a tri-phasic pattern, with breakpoints at 26 dpi (CI = 22.3, 29.2) and 130 dpi (CI = 121.0, 138.1). The study findings demonstrate that using bovine manure as BSAAO in soil enhances E. coli survival, regardless of strain, and adequate food safety practices are needed to reduce the risk of crop contamination. The findings of this study contribute data on E. coli concentrations in amended soils to assist stakeholders and regulators in making risk-based decisions on time intervals between the application of BSAAO and the production and harvest of fruits and vegetables.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Food Prot Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Food Prot Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos