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Chemistry-Enabled Intercellular Enzymatic Labeling for Monitoring the Immune Effects of Cytotoxic T Lymphocytes In Vivo.
He, Jiaqi; Liang, Chao; Yu, Xin-He; Ma, Xianbin; Qu, Yun; Zhuang, Wan-Ru; Li, Wenzhe; Nie, Weidong; Ren, Yue; Lei, Yao; Dong, Yuping; Xie, Hai-Yan.
Afiliación
  • He J; School of Life Science, Beijing Institute of Technology, Beijing 100081, P. R. China.
  • Liang C; School of Life Science, Beijing Institute of Technology, Beijing 100081, P. R. China.
  • Yu XH; State Key Laboratory of Green Pesticide, Central China Normal University, Wuhan, Hubei 430079, P. R. China.
  • Ma X; School of Medical Technology, Beijing Institute of Technology, Beijing 100081, P. R. China.
  • Qu Y; School of Life Science, Beijing Institute of Technology, Beijing 100081, P. R. China.
  • Zhuang WR; State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Chemical Biology Center, Peking University, Beijing 100191, P. R. China.
  • Li W; State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Chemical Biology Center, Peking University, Beijing 100191, P. R. China.
  • Nie W; School of Life Science, Beijing Institute of Technology, Beijing 100081, P. R. China.
  • Ren Y; Beijing Key Laboratory of Construction Tailorable Advanced Functional Materials and Green Applications, School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, P. R. China.
  • Lei Y; State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Chemical Biology Center, Peking University, Beijing 100191, P. R. China.
  • Dong Y; Beijing Key Laboratory of Construction Tailorable Advanced Functional Materials and Green Applications, School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, P. R. China.
  • Xie HY; State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Chemical Biology Center, Peking University, Beijing 100191, P. R. China.
Anal Chem ; 2024 Aug 14.
Article en En | MEDLINE | ID: mdl-39140208
ABSTRACT
Monitoring the effector function of cytotoxic T lymphocytes (CTLs) in vivo remains a great challenge. Here, we develop a chemistry-enabled enzymatic labeling approach to evaluate the tumor-specific immune response of CTLs by precisely monitoring the interaction between CTLs and tumor cells. Staphylococcus aureus sortase A (SrtA) is linked to the CTL surface through bioconjugate chemistry and then catalyzes the transfer of fluorescent-labeled substrate, 5-Tamra-LPETG, to CTLs. Meanwhile, the tumor cells are specifically decorated with N-terminal glycine residues (G5 peptide) through the inherent glycolmetabolism of cathepsin B-specific cleavable triacetylated N-azidoacetyl-d-mannosamine (CB-Ac3ManNAz) and click chemistry. After the infiltration of engineered CTLs into the tumor tissues, the immune-synapse-mediated specific interaction of CTLs and tumor cells leads to the accurate fluorescent labeling of tumor cells through the SrtA-catalyzed 5-Tamra-LPETG transfer. Therefore, the immune effect of CTLs as well as the performance of immune drugs can be determined, providing a novel strategy for pushing ahead immunotherapy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Anal Chem Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Anal Chem Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos