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Autophagy-induced cell death by aqueous and polyphenol-enriched extracts of honeybush (Cyclopia spp.) in liver and colon cancer cells.
Samodien, Sedicka; de Kock, Maryna; Joubert, Elizabeth; de Beer, Dalene; Kriel, Jurgen; Gelderblom, Wentzel C A; Lilly, Mariska.
Afiliación
  • Samodien S; Applied Microbial and Health Biotechnology Institute Cape Peninsula University of Technology Bellville South Africa.
  • de Kock M; Department of Medical Bioscience Program University of Western Cape Bellville South Africa.
  • Joubert E; Plant Bioactives Group, Post-Harvest & Agro-Processing Technologies Agricultural Research Council, Infruitec-Nietvoorbij Stellenbosch South Africa.
  • de Beer D; Department of Food Science Stellenbosch University Stellenbosch South Africa.
  • Kriel J; Plant Bioactives Group, Post-Harvest & Agro-Processing Technologies Agricultural Research Council, Infruitec-Nietvoorbij Stellenbosch South Africa.
  • Gelderblom WCA; Department of Food Science Stellenbosch University Stellenbosch South Africa.
  • Lilly M; Central Analytical Facilities, Electron Microscopy Unit Stellenbosch University Stellenbosch South Africa.
Food Sci Nutr ; 12(8): 5647-5662, 2024 Aug.
Article en En | MEDLINE | ID: mdl-39139978
ABSTRACT
The anti-cancer potential of Cyclopia species (honeybush) has been demonstrated in several models. The present study investigated the effects of aqueous and polyphenol-enriched (PE) extracts of C. subternata and C. genistoides, as well as mangiferin and hesperidin, on different cell growth parameters in human liver (HepG2) and colon (HT-29) cancer cells. Mangiferin and hesperidin were most abundant in C. genistoides and C. subternata, respectively. Cyclopia subternata extracts had the highest ferric-reducing antioxidant capacity. Following exposure of the cells to the extracts and compounds, cell viability, proliferation, and death (apoptosis and autophagy) were determined. Cyclopia subternata extracts reduced cell viability and inhibited cell proliferation the most, associated with depletion of ATP. In HepG2 cells, the PE extracts were less effective than the aqueous extracts in reducing cell viability but more effective in inhibiting cell proliferation. Despite disrupting cell growth, none of the extracts induced apoptosis. The aqueous extracts affected autophagy in both cancer cells. Disruption of mitochondrial membrane integrity by the different extracts, presumably via polyphenol/iron interactions, is postulated to be involved; however, mangiferin and hesperidin had no effect, suggesting that other polyphenols and/or complex interactions between compounds are likely responsible for the differential cytotoxic and/or cytoprotective effects of the extracts.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Food Sci Nutr Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Food Sci Nutr Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos