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In vitro assessment of the immunomodulatory effects of probiotic Bacillus strains on chicken PBMCs.
Larsberg, Filip; Sprechert, Maximilian; Hesse, Deike; Falker-Gieske, Clemens; Loh, Gunnar; Brockmann, Gudrun A; Kreuzer-Redmer, Susanne.
Afiliación
  • Larsberg F; Albrecht Daniel Thaer-Institute, Animal Breeding and Molecular Genetics, Humboldt-Universität zu Berlin, Berlin, Germany.
  • Sprechert M; Center for Animal Nutrition and Animal Welfare, Nutrigenomics, University of Veterinary Medicine Vienna, Vienna, Austria.
  • Hesse D; Albrecht Daniel Thaer-Institute, Animal Breeding and Molecular Genetics, Humboldt-Universität zu Berlin, Berlin, Germany.
  • Falker-Gieske C; Albrecht Daniel Thaer-Institute, Animal Breeding and Molecular Genetics, Humboldt-Universität zu Berlin, Berlin, Germany.
  • Loh G; Department of Animal Sciences, Georg-August-Universität, Göttingen, Germany.
  • Brockmann GA; Center for Integrated Breeding Research, Georg-August-Universität, Göttingen, Germany.
  • Kreuzer-Redmer S; Research, Development and Innovation, Nutrition and Care, Evonik Operations GmbH, Halle (Westfalen), Germany.
Front Immunol ; 15: 1415009, 2024.
Article en En | MEDLINE | ID: mdl-39139572
ABSTRACT
The beneficial effects of feeding probiotic Bacillus subtilis DSM 32315 (BS) and Bacillus velezensis CECT 5940 (BV) to chickens in vivo are well-documented, with potential immune modulation as a key mechanism. In this study, we investigated the direct interactions of chicken peripheral blood mononuclear cells (PBMCs) with BS or BV in vitro through whole transcriptome profiling and cytokine array analysis. Transcriptome profiling revealed 20 significantly differentially expressed genes (DEGs) in response to both Bacillus treatments, with twelve DEGs identified in BS-treated PBMCs and eight in BV-treated PBMCs. Pathway analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) indicated significant regulation of immune-related pathways by both BS and BV. Notably, BS treatment upregulated genes associated with immune cell surface markers (CD4, CD25, CD28), anti-inflammatory cytokine interleukin-10 (IL-10), and C-C motif chemokine ligand 5 (CCL5), while downregulating the gene encoding pro-inflammatory IL-16. BV treatment similarly affected genes associated with immune cell surface markers, IL-16, and CCL5, with no impact on the gene encoding IL-10. Both treatments induced higher expression of the gene encoding the avian ß-defensin 1 (AvBD1). The results of this in vitro study indicate an immunomodulatory effect of BS and BV in chicken PBMCs by regulating genes involved in anti-inflammatory, bacteriostatic, protective, and pro-inflammatory responses. Consequently, BS and BV may serve to augment the immune system's capacity to defend against infection by modulating immune responses and cytokine expression. Thus, the administration of these probiotics holds promise for reducing reliance on antimicrobials in farming practices.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus / Leucocitos Mononucleares / Pollos / Citocinas / Probióticos Límite: Animals Idioma: En Revista: Front Immunol Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus / Leucocitos Mononucleares / Pollos / Citocinas / Probióticos Límite: Animals Idioma: En Revista: Front Immunol Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza