Coupling of droplet-on-demand microfluidcs with ESI/MS to study single-cell catalysis.
RSC Adv
; 14(35): 25337-25346, 2024 Aug 12.
Article
en En
| MEDLINE
| ID: mdl-39139235
ABSTRACT
Droplet microfluidics provides an efficient method for analysing reactions within the range of nanoliters to picoliters. However, the sensitive, label-free and versatile detection with ESI/MS poses some difficulties. One challenge is the difficult association of droplets with the MS signal in high-throughput droplet analysis. Hence, a droplet-on-demand system for the generation of a few droplets can address this and other problems such as surfactant concentration or cross-contamination. Accordingly, the system has been further developed for online coupling with ESI/MS. To achieve this, we developed a setup enabling on-demand droplet generation by hydrodynamic gating, with downstream microscopic droplet detection and MS analysis. This facilitated the incorporation of 1-9 yeast cells into individual 1-5 nL droplets and the monitoring of yeast-catalysed transformation from ketoester to ethyl-3-hydroxybutyrate by MS. With our method a mean production rate of 0.035 ± 0.017 fmol per cell per h was observed with a detection limit of 0.30 µM. In conclusion, our droplet-on-demand method is a versatile and advantageous tool for cell encapsulation in droplets, droplet imaging and reaction detection using ESI/MS.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
RSC Adv
Año:
2024
Tipo del documento:
Article
Pais de publicación:
Reino Unido