The influence of various sample storage conditions and sample bacterial contamination on concentrations of routine biochemical parameters.

Gojkovic, Amara; Vladimirov, Sandra; Antonic, Tamara; Bogavac-Stanojevic, Natasa; Novovic, Katarina; Spasojevic-Kalimanovska, Vesna; Filipic, Brankica

Gojkovic, Amara; Vladimirov, Sandra; Antonic, Tamara; Bogavac-Stanojevic, Natasa; Novovic, Katarina; Spasojevic-Kalimanovska, Vesna; Filipic, Brankica.

Afiliación

Gojkovic A; University of Belgrade, Faculty of Pharmacy, Department of Medical Biochemistry, Belgrade.
Vladimirov S; University of Belgrade, Faculty of Pharmacy, Department of Medical Biochemistry, Belgrade.
Antonic T; University of Belgrade, Faculty of Pharmacy, Department of Medical Biochemistry, Belgrade.
Bogavac-Stanojevic N; University of Belgrade, Faculty of Pharmacy, Department of Medical Biochemistry, Belgrade.
Novovic K; University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Belgrade.
Spasojevic-Kalimanovska V; University of Belgrade, Faculty of Pharmacy, Department of Medical Biochemistry, Belgrade.
Filipic B; University of Belgrade, Faculty of Pharmacy, Department of Microbiology and Immunology, Belgrade.

J Med Biochem ; 43(4): 413-423, 2024 Jun 15.

Article en En | MEDLINE | ID: mdl-39139161

ABSTRACT

ABSTRACT

Background:

The pre-analytical (PA) phase is the most vulnerable phase of the laboratory testing procedure, with critical procedures-collection, handling, sample transport, and time and temperature of sample storage. This study aimed to examine the stability of basic biochemical parameters depending on the samples' storage conditions and the number of freeze-thaw cycles (FTCs). In parallel, the presence of sample bacterial contamination during routine laboratory work was examined.

Methods:

Two plasma pools (ethylenediaminetetraacetic acid (EDTA), and sodium-fluoride/potassium oxalate plasma (NaF)) were stored at +4 ËC/-20 ËC. Total chole - sterol (TC), glucose, triglycerides (TG), urea, and albumin concentrations were measured using BioSystems reagents (cholesterol oxidase/peroxidase, glucose oxidase/per - oxidase, glycerol phosphate oxidase/peroxidase, urease/ salicylate, and bromcresol green method, respectively) on Ilab 300+. Sample bacterial contamination was determined by 16S rRNA sequence analysis. The expe - riment encompassed a 5 day-period Day 1-fresh sample, Day 2-1st FTC, Day 3-2nd FTC, Day 4-3rd FTC, Day 5-4th FTC. The appearance of bacteria in two consecutive samples was the experiment's endpoint.

Palabras clave

bacterial cross-contamination; freeze-thaw cycles; pre-analytical phase; preservative potassium-fluoride

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Med Biochem Año: 2024 Tipo del documento: Article Pais de publicación: