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Megakaryocyte maturation involves activation of the adaptive unfolded protein response.
Faiz, M; Kalev-Zylinska, M L; Dunstan-Harrison, C; Singleton, D C; Hay, M P; Ledgerwood, E C.
Afiliación
  • Faiz M; Department of Biochemistry, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand.
  • Kalev-Zylinska ML; Blood and Cancer Biology Laboratory, Department of Molecular Medicine & Pathology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
  • Dunstan-Harrison C; Department of Biochemistry, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand.
  • Singleton DC; Auckland Cancer Society Research Centre, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
  • Hay MP; Auckland Cancer Society Research Centre, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
  • Ledgerwood EC; Department of Biochemistry, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand.
Genes Cells ; 2024 Aug 13.
Article en En | MEDLINE | ID: mdl-39138929
ABSTRACT
Endoplasmic reticulum stress triggers the unfolded protein response (UPR) to promote cell survival or apoptosis. Transient endoplasmic reticulum stress activation has been reported to trigger megakaryocyte production, and UPR activation has been reported as a feature of megakaryocytic cancers. However, the role of UPR signaling in megakaryocyte biology is not fully understood. We studied the involvement of UPR in human megakaryocytic differentiation using PMA (phorbol 12-myristate 13-acetate)-induced maturation of megakaryoblastic cell lines and thrombopoietin-induced differentiation of human peripheral blood-derived progenitors. Our results demonstrate that an adaptive UPR is a feature of megakaryocytic differentiation and that this response is not associated with ER stress-induced apoptosis. Differentiation did not alter the response to the canonical endoplasmic reticulum stressors DTT or thapsigargin. However, thapsigargin, but not DTT, inhibited differentiation, consistent with the involvement of Ca2+ signaling in megakaryocyte differentiation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Genes Cells Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Genes Cells Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Reino Unido