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Mechanism of action and synergistic effect of Eugenia uniflora extract in Candida spp.
Souza, Luanda B F C; de Oliveira Bento, Aurélio; Lourenço, Estela M G; Ferreira, Magda R A; Oliveira, Wogenes N; Soares, Luiz Alberto L; G Barbosa, Euzébio; Rocha, Hugo A O; Chaves, Guilherme Maranhão.
Afiliación
  • Souza LBFC; Medical and Molecular Mycology Laboratory, Department of Clinical and Toxicological Analyses, Federal University of Rio Grande do Norte, Natal, Brazil.
  • de Oliveira Bento A; Medical and Molecular Mycology Laboratory, Department of Clinical and Toxicological Analyses, Federal University of Rio Grande do Norte, Natal, Brazil.
  • Lourenço EMG; Department of Pharmacy, Federal University of Rio Grande do Norte, Natal, Brazil.
  • Ferreira MRA; Laboratory of Synthesis and Transformation of Organic Molecules, LP4, Institute of Chemistry, Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil.
  • Oliveira WN; Department of Pharmaceutical Sciences, Federal University of Pernambuco, Recife, Brazil.
  • Soares LAL; Department of Pharmacy, Federal University of Rio Grande do Norte, Natal, Brazil.
  • G Barbosa E; Department of Pharmaceutical Sciences, Federal University of Pernambuco, Recife, Brazil.
  • Rocha HAO; Department of Pharmaceutical Sciences, Federal University of Pernambuco, Recife, Brazil.
  • Chaves GM; Department of Biochemistry, Federal University of Rio Grande do Norte, Natal, Brazil.
PLoS One ; 19(8): e0303878, 2024.
Article en En | MEDLINE | ID: mdl-39137202
ABSTRACT
The limited arsenal of antifungal drugs have prompted the search for novel molecules with biological activity. This study aimed to characterize the antifungal mechanism of action of Eugenia uniflora extract and its synergistic activity with commercially available antifungal drugs on the following Candida species C. albicans, C. tropicalis, C. glabrata, C. parapsilosis and C. dubliniensis. In silico analysis was performed to predict antifungal activity of the major compounds present in the extract. Minimal inhibitory concentrations (MICs) were determined in the presence of exogenous ergosterol and sorbitol. Yeast cells were grown in the presence of stressors. The loss of membrane integrity was assessed using propidium iodide staining (fluorescence emission). Synergism between the extract and antifungal compounds (in addition to time kill-curves) was determined. Molecular docking revealed possible interactions between myricitrin and acid gallic and enzymes involved in ergosterol and cell wall biosynthesis. Candida cells grown in the presence of the extract with addition of exogenous ergosterol and sorbitol showed 2 to 8-fold increased MICs. Strains treated with the extract revealed greater loss of membrane integrity when compared to their Fluconazole counterparts, but this effect was less pronounced than the membrane damage caused by Amphotericin B. The extract also made the strains more susceptible to Congo red and Calcofluor white. A synergistic action of the extract with Fluconazole and Micafungin was observed. The E. uniflora extract may be a viable option for the treatment of Candida infections.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Candida / Extractos Vegetales / Pruebas de Sensibilidad Microbiana / Sinergismo Farmacológico / Eugenia / Antifúngicos Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Candida / Extractos Vegetales / Pruebas de Sensibilidad Microbiana / Sinergismo Farmacológico / Eugenia / Antifúngicos Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos