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Mitochondrial dysfunction in kidney stones and relief of kidney stones after reducing mtROS.
Xu, Yuexian; Li, Guoxiang; Ge, Defeng; Chen, Yan; Hou, Bingbing; Hao, Zongyao.
Afiliación
  • Xu Y; Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.
  • Li G; Institute of Urology, Anhui Medical University, Hefei, China.
  • Ge D; Anhui Province Key Laboratory of Urological and Andrological Diseases Research and Medical Transformation, Anhui Medical University, Hefei, China.
  • Chen Y; Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.
  • Hou B; Institute of Urology, Anhui Medical University, Hefei, China.
  • Hao Z; Anhui Province Key Laboratory of Urological and Andrological Diseases Research and Medical Transformation, Anhui Medical University, Hefei, China.
Urolithiasis ; 52(1): 117, 2024 Aug 13.
Article en En | MEDLINE | ID: mdl-39136789
ABSTRACT
Mitochondria are essential organelles because they generate the energy required for cellular functions. Kidney stones, as one of the most common urological diseases, have garnered significant attention. In this study, we first collected peripheral venous blood from patients with kidney stones and used qRT-PCR to detect mitochondrial DNA (mtDNA) copy number as a means of assessing mitochondrial function in these patients. Subsequently, through Western blotting, qPCR, immunofluorescence, immunohistochemistry, and transmission electron microscopy, we examined whether calcium oxalate crystals could cause mitochondrial dysfunction in the kidney in both in vitro and in vivo. We then examined the intersection of the DEGs obtained by transcriptome sequencing of the mouse kidney stone model with mitochondria-related genes, and performed KEGG and GO analyses on the intersecting genes. Finally, we administered the mitochondrial ROS scavenger Mito-Tempo in vivo and observed its effects. Our findings revealed that patients with kidney stones had a reduced mtDNA copy number in their peripheral venous blood compared to the control group, suggesting mitochondrial dysfunction in this population. This conclusion was further validated through in vitro and in vivo experiments. Enrichment analyses revealed that the intersecting genes were closely related to metabolism. We observed that after mitochondrial function was preserved, the deposition of calcium oxalate crystals decreased, and the kidney damage and inflammation caused by them were also alleviated. Our research indicates that kidney stones can cause mitochondrial dysfunction. After clearing mtROS, the damage and inflammation caused by kidney stones are reversed, providing new insights into the prevention and treatment of kidney stones.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxalato de Calcio / ADN Mitocondrial / Cálculos Renales / Especies Reactivas de Oxígeno / Mitocondrias Límite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: Urolithiasis Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxalato de Calcio / ADN Mitocondrial / Cálculos Renales / Especies Reactivas de Oxígeno / Mitocondrias Límite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: Urolithiasis Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania