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Study on Active Components and Mechanism of Lettuce Latex Against Spodoptera Litura.
Gu, Chengzhen; Zeng, Bixue; Wang, Mengmeng; Zhang, Yujia; Yan, Chengxi; Lin, Yangzheng; Khan, Afsar; Zeng, Rensen; Song, Yuanyuan.
Afiliación
  • Gu C; Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
  • Zeng B; Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
  • Wang M; Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
  • Zhang Y; Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
  • Yan C; Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
  • Lin Y; Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
  • Khan A; Department of Chemistry, COMSATS University Islamabad, Abbottabad Campus, Abbottabad, 22060, Pakistan.
  • Zeng R; Laboratory of Ministry of Agriculture and Rural Affairs of Biological Breeding for Fujian and Taiwan Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
  • Song Y; Laboratory of Ministry of Agriculture and Rural Affairs of Biological Breeding for Fujian and Taiwan Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Chem Biodivers ; : e202400993, 2024 Aug 13.
Article en En | MEDLINE | ID: mdl-39136195
ABSTRACT
Plant latex is a sticky emulsion exuded from laticifers once the plant is damaged. Latex is an essential component of plant defense against herbivores. Lettuce (Lactuca sativa L.) in the Compositae family has relatively fewer insect herbivores compared with other leaf vegetables. The larvae of a generalist lepidopteran pest Spodoptera litura (Fabricius) avoided feeding on living lettuce plants. However, the larvae rapidly damaged the excised leaves that were unable to produce latex. Six compounds were isolated from lettuce latex. They were identified as 2,5-dihydroxybenzaldehyde (1), 3ß-hydroxy-4,15-dehydrograndolide (2), annuolide D (3), lactucin (4), lactucopicrin (5), and hanphyllin (6). Bioassays showed that the inhibition rate of compound 1 (2,5-dihydroxybenzaldehyde) and 6 (hanphyllin, a sesquiterpene lactone) on the weight gain of S. litura were 52.4 % and 10 %, respectively, at the concentration of 100 µg/g. RNA-seq analyses showed that larval exposure to compound 1 down-regulated the genes associated with heterobiotic metabolism including drug metabolism-cytochrome P450, metabolism of xenobiotics by cytochrome P450, retinol metabolism, glutathione metabolism, and drug metabolism-other enzymes (mainly uridine diphosphate glucuronyltransferase, UGTs). RT-qPCR further confirmed that 33 genes in the family of carboxylesterase (CarE), P450s and UGTs were down-regulated by compound 1. The activities of CarE, P450s and UGTs in the larvae fed on diets containing compound 1 were significantly lower than those fed on control diets, with the inhibition for the three detoxification enzymes being 55.4 %, 53.9 %, and 52.9 %. These findings suggest that secondary metabolites including 2,5-dihydroxybenzaldehyde in the latex play a key role in protecting lettuce from insect herbivory.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Chem Biodivers Asunto de la revista: BIOQUIMICA / QUIMICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Chem Biodivers Asunto de la revista: BIOQUIMICA / QUIMICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza