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Nattokinase's Neuroprotective Mechanisms in Ischemic Stroke: Targeting Inflammation, Oxidative Stress, and Coagulation.
Yang, Xin-Ying; Wang, Sheng-Lin; Xue, Wen-Chi; Zhang, Yu-Peng; Li, Liang-Liang; Luo, Zhao-Hu; Zhang, Feng-Jiao.
Afiliación
  • Yang XY; Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, China.
  • Wang SL; Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, China.
  • Xue WC; Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, China.
  • Zhang YP; Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, China.
  • Li LL; Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, China.
  • Luo ZH; Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, China.
  • Zhang FJ; Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, China.
Antioxid Redox Signal ; 2024 Sep 05.
Article en En | MEDLINE | ID: mdl-39135387
ABSTRACT

Aims:

Nattokinase (NK), a potent serine endopeptidase, has exhibited a variety of pharmacological effects, including thrombolysis, anti-inflammation, and antioxidative stress. Building on previous research highlighting NK's promise in nerve regeneration, our study investigated whether NK exerted protective effects in transient middle cerebral artery occlusion (tMCAO)-induced cerebral ischemia-reperfusion injury and the underlying mechanisms.

Results:

The rats were administered NK (5000, 10000, 20000 FU/kg, i.g., 7 days before surgery, once daily). We showed that NK treatment dose dependently reduced the infarction volume and improved neurological symptoms, decreased the proinflammatory and coagulation cytokines levels, and attenuated reactive oxygen species (ROS) in the infarcted area of tMCAO rats. We also found that NK could exert neuroprotective effects in a variety of vitro models, including the microglia inflammation model and neuronal oxygen-glucose deprivation/reperfusion (OGD/R) model. Notably, NK effectively countered OGD/R-induced neuron death, modulating diverse pathways, including autophagy, apoptosis, PARP-dependent death, and endoplasmic reticulum stress. Furthermore, the neuroprotection of NK was blocked by phenylmethylsulfonyl fluoride (PMSF), a serine endopeptidase inhibitor. We revealed that heat-inactive NK was unable to protect against tMCAO injury and other vitro models, suggesting NK attenuated ischemic injury by its enzymatic activity. We conducted a proteomic analysis and found inflammation and coagulation were involved in the occurrence of tMCAO model and in the therapeutic effect of NK. Innovation and

Conclusion:

In conclusion, these data demonstrated that NK had multifaceted neuroprotection in ischemic brain injury, and the therapeutic effect of NK was related with serine endopeptidase activity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Antioxid Redox Signal Asunto de la revista: METABOLISMO Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Antioxid Redox Signal Asunto de la revista: METABOLISMO Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos