Your browser doesn't support javascript.
loading
The pharmacokinetics/pharmacodynamics integration of tilmicosin against Mycoplasma synoviae in vitro and in vivo.
Yan, Xiu; Liu, Jinxin; Li, Weihuo; Song, Shuti; Yao, Zhaofeng; Jia, Yixin; Yuan, Sheng; Yang, Hong; Zhang, Nan.
Afiliación
  • Yan X; School of Life Science and Engineering, Foshan University, Foshan, China.
  • Liu J; Laboratory Animal Center, Peking University Shenzhen Graduate School, Shenzhen, China.
  • Li W; School of Life Science and Engineering, Foshan University, Foshan, China.
  • Song S; School of Life Science and Engineering, Foshan University, Foshan, China.
  • Yao Z; School of Life Science and Engineering, Foshan University, Foshan, China.
  • Jia Y; School of Life Science and Engineering, Foshan University, Foshan, China.
  • Yuan S; School of Life Science and Engineering, Foshan University, Foshan, China.
  • Yang H; School of Life Science and Engineering, Foshan University, Foshan, China.
  • Zhang N; School of Life Science and Engineering, Foshan University, Foshan, China.
J Vet Pharmacol Ther ; 2024 Aug 12.
Article en En | MEDLINE | ID: mdl-39135274
ABSTRACT
Mycoplasma synoviae (MS) infection is a serious threat to poultry industry in China. Tilmicosin is a semisynthetic macrolide antibiotic used only in animals and has shown potential efficacy against MS, but there were no reported articles concerning the pharmacokinetics/pharmacodynamics (PK/PD) interactions of tilmicosin against MS in vitro and vivo. This study aimed to assess the antibacterial activity of tilmicosin against MS in vitro and in vivo using PK/PD model to provide maximal efficacy. The minimum inhibitory concentration (MIC) and killing rates of different drug concentrations were measured using the microdilution method in vitro. Then, tilmicosin was administered orally to the MS-infected chickens at doses of 7.5 and 60 mg/kg, and the PK parameters of tilmicosin in joint dialysates were determined using high-pressure liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) combined with the microdialysis technique. The antibacterial effect (△E) was calculated when the infected chickens were administered a single oral dose of tilmicosin at 4, 7.5, 15, 30, and 60 mg/kg b.w. The PK and PD data were fitted using the Sigmoid Emax model to evaluate the PK/PD interactions of tilmicosin against MS. The bactericidal activity of tilmicosin against MS was concentration dependent. Furthermore, the PK/PD index of AUC0-72h/MIC exhibited the most optimal fitting results (R2 = .98). The MS load decreased by 1, 2, and 3 Log10 CFU/mL, then AUC/MIC was determined as 13.99, 20.53, and 28.23 h, respectively, and the bactericidal effect can be achieved when the dose of MS-infected chickens is at 31.64 mg/kg b.w. The findings of this study hold significant implications for optimizing the treatment regimen for MS infection.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Vet Pharmacol Ther Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Vet Pharmacol Ther Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido