Hybrid chain reaction nanoscaffold-based functional nucleic acid nanomaterial cascaded with rolling circle amplification for signal enhanced miRNA let-7a detection.

Jiang, Hao; Peng, Zhao; Lv, Xuefei; Liu, Ying; Li, Xiaoqiong; Deng, Yulin

Jiang, Hao; Peng, Zhao; Lv, Xuefei; Liu, Ying; Li, Xiaoqiong; Deng, Yulin.

Afiliación

Jiang H; Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Medical Technology, Beijing Institute of Technology, Beijing, 100081, China.
Peng Z; Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Medical Technology, Beijing Institute of Technology, Beijing, 100081, China.
Lv X; Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Medical Technology, Beijing Institute of Technology, Beijing, 100081, China. xuefeilv@163.com.
Liu Y; Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Medical Technology, Beijing Institute of Technology, Beijing, 100081, China.
Li X; Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Medical Technology, Beijing Institute of Technology, Beijing, 100081, China.
Deng Y; Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Medical Technology, Beijing Institute of Technology, Beijing, 100081, China.

Mikrochim Acta ; 191(9): 533, 2024 08 13.

Article en En | MEDLINE | ID: mdl-39134753

ABSTRACT

ABSTRACT

A novel functional nucleic acid (FNA) nanomaterial based on hybrid chain reaction (HCR) nanoscaffolds is proposed to solve the problem of time superposition and repeated primer design in sensitive miRND detection using cascade amplification technique. Rolling circle amplification (RCA) was cascaded with the prepared FNA nanomaterials for miRNA let-7a (as a model target) sensitive detection by lateral flow assay (LFA). Under the optimal conditions, the proposed RCA-FNA-LFA assay demonstrated the specificity and accuracy for miRNA let-7a detection with a detection limit of 1.07 pM, which increased sensitivity by nearly 20 times compared with that of RCA -LFA assay. It is worth noting that the non-target-dependent self-assembly process of HCR nanoscaffolds does not take up the whole detection time, thus, less time is taken than that of the conventional cascaded method. Moreover, the proposed assay does not need to consider the system compatibility between two kinds of isothermal amplification techniques. As for detection of different miRNAs, only the homologous arm of the padlock probe of RCA needs to be changed, while the FNA nanomaterial does not need any change, which greatly simplifies the primer design of the cascaded amplification techniques. With further development, the proposed RCA-FNA-LFA assay might achieve more sensitive and faster results to better satisfy the requirements of clinical diagnosis combing with more sensitive labels or small strip reader.

Asunto(s)

Límite de Detección; MicroARNs; Nanoestructuras; Técnicas de Amplificación de Ácido Nucleico; Técnicas de Amplificación de Ácido Nucleico/métodos; MicroARNs/análisis; Humanos; Nanoestructuras/química; Técnicas Biosensibles/métodos

Palabras clave

Cascaded amplification; Functional nucleic acid; Hybrid chain reaction; Lateral flow assay; MiRNA let 7a; Nanoscaffold; Rolling circle amplification

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Amplificación de Ácido Nucleico / MicroARNs / Nanoestructuras / Límite de Detección Límite: Humans Idioma: En Revista: Mikrochim Acta Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Austria

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