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Characterization, whole-genome sequence analysis, and protease production of a new thermophilic Bacillus licheniformis strain isolated from Debagh hot spring, Algeria.
Boukeroui, Yasmina; González-Siso, María-Isabel; DeCastro, María-Eugenia; Arab, Mounia; Aissaoui, Nadia; Nas, Fatima; Saibi, Amina Nour Elhouda; Klouche Khelil, Nihel.
Afiliación
  • Boukeroui Y; Laboratory of Applied Microbiology in Food, Biomedical and Environment (LAMAABE Laboratory), Department of Biology, Faculty of Nature and Life, Earth and Universe Sciences, University of Tlemcen, 13000, Tlemcen, Algeria.
  • González-Siso MI; Grupo EXPRELA, Centro Interdisciplinar de Química E Bioloxía (CICA), Facultade de Ciencias, Universidade da Coruña, 15071 , A Coruña, Spain.
  • DeCastro ME; Grupo EXPRELA, Centro Interdisciplinar de Química E Bioloxía (CICA), Facultade de Ciencias, Universidade da Coruña, 15071 , A Coruña, Spain.
  • Arab M; Laboratory of Applied Microbiology in Food, Biomedical and Environment (LAMAABE Laboratory), Department of Biology, Faculty of Nature and Life, Earth and Universe Sciences, University of Tlemcen, 13000, Tlemcen, Algeria.
  • Aissaoui N; Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene, 16000, Algiers, Algeria.
  • Nas F; Laboratory of Sustainable Management of Natural Resources in Arid and Semi Arid Areas (GDRN), Institute of Sciences, University Center of Naâma, 45000, Naâma, Algeria.
  • Saibi ANE; Laboratory of Applied Microbiology in Food, Biomedical and Environment (LAMAABE Laboratory), Department of Biology, Faculty of Nature and Life, Earth and Universe Sciences, University of Tlemcen, 13000, Tlemcen, Algeria.
  • Klouche Khelil N; Laboratory of Applied Microbiology in Food, Biomedical and Environment (LAMAABE Laboratory), Department of Biology, Faculty of Nature and Life, Earth and Universe Sciences, University of Tlemcen, 13000, Tlemcen, Algeria.
Int Microbiol ; 2024 Aug 12.
Article en En | MEDLINE | ID: mdl-39129036
ABSTRACT
A new thermophilic strain, designated as Bacillus sp. LMB3902, was isolated from Hammam Debagh, the hottest spring in Algeria (up to 98 °C). This isolate showed high protease production in skim milk media at 55 °C and exhibited significant specific protease activity by using azocasein as a substrate (157.50 U/mg). Through conventional methods, chemotaxonomic characteristics, 16S rRNA gene sequencing, and comparative genomic analysis with the closely related strain Bacillus licheniformis DSM 13 (ATCC 14580 T), the isolate Bacillus sp. LMB3902 was identified as a potentially new strain of Bacillus licheniformis. In addition, the gene functions of Bacillus sp. LMB3902 strain were predicted using the Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, Clusters of Orthologous Groups, Non-Redundant Protein Sequence Database, Swiss-Prot, and Pfam databases. The results showed that the genome size of Bacillus sp. LMB3902 was 4.279.557 bp, with an average GC content of 46%. The genome contained 4.760 predicted genes, including 8 rRNAs, 78 tRNAs, and 24 sRNAs. A total of 235 protease genes were annotated including 50 proteases with transmembrane helix structures and eight secreted proteases with signal peptides. Additionally, the majority of secondary metabolites found by antiSMASH platform showed low similarity to identified natural products, such as fengicin (53%), lichenysin (57%), and surfactin (34%), suggesting that this strain may encode for novel uncharacterized natural products which can be useful for biotechnological applications. This study is the first report that describes the complete genome sequence, taxono-genomics, and gene annotation as well as protease production of the Bacillus genus in this hydrothermal vent.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Int Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Argelia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Int Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Argelia Pais de publicación: Suiza