Your browser doesn't support javascript.
loading
Combined alkali-photocatalytic stimulation enables click microbial domestication for boosted ammonia nitrogen removal.
Sun, Zhen; Du, Mingzhu; Yao, Zongli; Wang, Ming; Gao, Pengcheng; Liu, Nian; Liu, Qinhong; Kang, Shifei; Lai, Qifang.
Afiliación
  • Sun Z; East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Shanghai 200093, PR China.
  • Du M; Department of Environmental Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, PR China; Institute of Photochemistry and Photofunctional Materials (IPPM), University of Shanghai for Science and Technology, Shanghai 200093, PR China.
  • Yao Z; East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Shanghai 200093, PR China.
  • Wang M; Department of Environmental Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, PR China.
  • Gao P; East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Shanghai 200093, PR China.
  • Liu N; Department of Environmental Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, PR China; Institute of Photochemistry and Photofunctional Materials (IPPM), University of Shanghai for Science and Technology, Shanghai 200093, PR China.
  • Liu Q; Department of Environmental Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, PR China.
  • Kang S; Department of Environmental Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, PR China; Institute of Photochemistry and Photofunctional Materials (IPPM), University of Shanghai for Science and Technology, Shanghai 200093, PR China. Electronic address: sfkan
  • Lai Q; East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Shanghai 200093, PR China. Electronic address: laiqf@ecsf.ac.cn.
J Hazard Mater ; 478: 135417, 2024 Oct 05.
Article en En | MEDLINE | ID: mdl-39128151
ABSTRACT
Microbe-driven ammonia nitrogen removal plays a crucial role in the nitrogen cycle and wastewater treatment. However, the rational methods and mechanisms for boosting nitrogen conversion through microbial domestication are still limited. Herein, a combined alkali-photocatalytic stimulation strategy was developed to activate the Halomonas shizuishanensis DWK9 for efficient ammonia nitrogen removal. The strain DWK9 selected from saline-alkaline soil in Northwestern China possessed strong resistance to stress of saline-alkaline environment and free radicals, and was abundant in nitrogen conversion genes, thus is an ideal model for advanced microbial domestication. Bacterial in the combined alkali-photocatalytic stimulation group achieved a high ammonia nitrogen conversion rate of 67.5 %, 10 times outperforming the non-stimulated and single alkali/photocatalytic stimulation control groups. Morphology analysis revealed that the bacteria in the alkali-photocatalytic stimulated group formed a favorable structure for bioelectric transfer. Remarkably, the domesticated bacteria demonstrated improved electrochemical properties, including increased current capacity and lower overpotentials and impedance. Prokaryotic transcription genetic analysis together with qPCR analysis showed upregulation of denitrification-related metabolic pathway genes. A novel FAD dependent and NAD(P)H independent energy mode has been proposed. The universality and effectiveness of the as-developed combined alkali-photocatalytic microbial domestication strategy were further validated through indicator fish survival experiments. This work provides unprecedented degrees of freedom for the exploration of rational microbial engineering for optimized and controllable biogeochemical conversion.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Halomonas / Álcalis / Amoníaco / Nitrógeno Idioma: En Revista: J Hazard Mater Asunto de la revista: SAUDE AMBIENTAL Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Halomonas / Álcalis / Amoníaco / Nitrógeno Idioma: En Revista: J Hazard Mater Asunto de la revista: SAUDE AMBIENTAL Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos