Real-Time RT-PCR for the Diagnosis of Epizootic Hemorrhagic Disease Virus.
Methods Mol Biol
; 2838: 155-161, 2024.
Article
en En
| MEDLINE
| ID: mdl-39126630
ABSTRACT
Real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) has become an essential tool in rapid and reliable detection of animal diseases such as epizootic hemorrhagic disease (EHD). Here we provide a protocol for the detection of epizootic hemorrhagic disease virus (EHDV) genetic material in blood and tissue samples, using a real-time RT-PCR that targets a conserved region in segment 9 of the EHDV genome. This protocol can be used to detect up to approximately 90 samples in a single run and can be completed in less than 4 h.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus de la Enfermedad Hemorrágica Epizoótica
/
Infecciones por Reoviridae
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Reacción en Cadena en Tiempo Real de la Polimerasa
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2024
Tipo del documento:
Article
País de afiliación:
Reino Unido
Pais de publicación:
Estados Unidos