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In vitro dissolution of encapsulated or dispersed Vitamin e and cholesterol is correlated with preservation of refrigerated ovine sperm.
Taouzinet, L; Fatmi, S; Khellouf, A; Benberkane, A; Kribeche, A; Toutou, Z; Skiba, M; Iguer-Ouada, M.
Afiliación
  • Taouzinet L; Centre de Recherche en Technologies Agroalimentaires, Route de Targa Ouzemmour, Campus Universitaire; Associated Laboratory in Marine Ecosystems and Aquaculture, Faculty of Nature and Life Sciences, Universite de Bejaia, Bejaia 06000, Algeria. lamia.taouzinet@crtaa.univ-bejaia.dz.
  • Fatmi S; Associated Laboratory in Marine Ecosystems and Aquaculture, Faculty of Nature and Life Sciences; Technology Pharmaceutical Laboratory, Department of Processes Engineering, Faculty of Technology, Universite de Bejaia, Bejaia 06000, Algeria. UNIROUEN, DC2N INSERM U1239-Galenic Pharmaceutical Team, UFR
  • Khellouf A; Associated Laboratory in Marine Ecosystems and Aquaculture, Faculty of Nature and Life Sciences, Universite de Bejaia, Bejaia 06000, Algeria.
  • Benberkane A; Associated Laboratory in Marine Ecosystems and Aquaculture, Faculty of Nature and Life Sciences, Universite de Bejaia, Bejaia 06000, Algeria.
  • Kribeche A; Laboratoire de Biomathematique, Biochimie, Biophysique Et Scientometrie, Faculte des Sciences de la Nature Et de la Vie, Universite de Bejaia, Bejaia 06000, Algeria.
  • Toutou Z; Technology Pharmaceutical Laboratory, Department of Processes Engineering, Faculty of Technology; LMPA, Department of Process Engineering, Faculty of Technology, Universite de Bejaia, Bejaia 06000, Algeria.
  • Skiba M; UNIROUEN, DC2N INSERM U1239-Galenic Pharmaceutical Team, UFR of Health, Normandy University, 22 Bd Gambetta, Rouen, France.
  • Iguer-Ouada M; Associated Laboratory in Marine Ecosystems and Aquaculture, Faculty of Nature and Life Sciences, Universite de Bejaia, Bejaia 06000, Algeria.
Cryo Letters ; 45(5): 294-300, 2024.
Article en En | MEDLINE | ID: mdl-39126331
ABSTRACT

BACKGROUND:

Vitamin E ( -tocopherol) and cholesterol are crucial components in cellular protection and physiological processes. Their uses in biological media face challenges due to their poor solubility and stability.

OBJECTIVE:

The study investigated the complex interactions of these bioactive compounds in various encapsulation systems of cyclodextrin and liposome, as well as dispersion in PEG-6000, in an attempt to improve the viability, motility, and preservation of ovine sperm cells. MATERIALS AND

METHODS:

The work explored the in vitro dissolution kinetics of vitamin E (d-tocopherol) and cholesterol using semi-empirical models.

RESULTS:

The release profiles of VitE and Chl varied considerably, depending on the specific carrier systems. For liposome-loaded VitE and Chl, the Korsmeyer-Peppas model gave the best fit; for CD/VitE and CD/Chl, the Higuchi model provided the best fit, whereas for PEG-6000 dispersions (VitE and Chl) both the Higuchi and Korsmeyer-Peppas models demonstrated the excellent fit. All systems indicated a Fickian diffusion mechanism dictated by the concentration gradient. The delivery of VitE and Chl with CD, liposome and PEG dispersion significantly increased sperm mobility and motility. The effect on the VCL parameter was the greatest by liposome-loaded VitE and Chl, followed by CD encapsulation and PEG-6000 dispersion.

CONCLUSION:

The dynamics of vitamin E and cholesterol within innovative delivery systems offers valuable insights into the development of advanced solutions in reproductive health, particularly on improving the viability, motility of refrigerated ovine sperm cells. Doi.org/10.54680/fr24510110712.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de Semen / Motilidad Espermática / Espermatozoides / Vitamina E / Colesterol / Liposomas Límite: Animals Idioma: En Revista: Cryo Letters Asunto de la revista: BIOLOGIA / QUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Argelia Pais de publicación: Reino Unido
Buscar en Google
Añadir a Mi BVS
Imprimir
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PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de Semen / Motilidad Espermática / Espermatozoides / Vitamina E / Colesterol / Liposomas Límite: Animals Idioma: En Revista: Cryo Letters Asunto de la revista: BIOLOGIA / QUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Argelia Pais de publicación: Reino Unido