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Effect of different concentrations of inulin on ram sperm quality during cryopreservation.
Rahimi, Z; Haiarian, H; Karamishabankareh, H; Soltani, L.
Afiliación
  • Rahimi Z; Department of Animal Science, Faculty of Agricultural and Engineering Science, Razi University, Kermanshah.
  • Haiarian H; Department of Animal Science, Faculty of Agricultural and Engineering Science, Razi University, Kermanshah, Iran. h.hajarian@razi.ac.ir.
  • Karamishabankareh H; Department of Animal Science, Faculty of Agricultural and Engineering Science, Razi University, Kermanshah, Iran.
  • Soltani L; Department of Animal Science, Faculty of Agricultural and Engineering Science, Razi University, Kermanshah, Iran.
Cryo Letters ; 45(5): 288-293, 2024.
Article en En | MEDLINE | ID: mdl-39126330
ABSTRACT

BACKGROUND:

In reproductive biotechnology, sperm cryopreservation has a vital role to play. Cryopreservation of sperm produces reactive oxygen species (ROS), which disrupt sperm function and structural competence. Numerous protective chemicals, including fructans, have been used during sperm cryopreservation.

OBJECTIVES:

To evaluate the effect of different concentrations of the fructosan inulin on ram sperm quality parameters, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) production after freezing and thawing. MATERIALS AND

METHODS:

The pooled samples from four healthy rams were divided into seven equal aliquots and diluted in a Tris-base extender supplemented with 1, 2, 4, 8, 16, and 28 mM of inulin or without inulin supplementation (control). By using liquid nitrogen vapor, the semen was frozen and stored at 196 degree C.

RESULTS:

The total motility, viability, and DNA integrity were significantly improved after freeze-thawing with 28 mM inulin, compared to other treatment groups (P < 0.05). A Tris-based extender containing 16 and 28 mM of inulin displayed the highest levels of ram sperm membrane integrity when compared with the control (p <0.05). The abnormality of ram sperm was increased during freeze-thawing at control and 1 mM of inulin, compared to 16 and 28 mM of inulin (P < 0.05). Additionally, 28 mM of inulin decreased MDA and increased SOD activity in ram sperm in comparison with the other treatments (P < 0.05).

CONCLUSION:

As a result, 28 mM of inulin could be beneficial for the cryopreservation industry and reduce the harmful effects of freeze-thawing on ram sperm. Doi.org/10.54680/fr24510110512.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de Semen / Motilidad Espermática / Espermatozoides / Superóxido Dismutasa / Criopreservación / Crioprotectores / Inulina / Malondialdehído Límite: Animals Idioma: En Revista: Cryo Letters Asunto de la revista: BIOLOGIA / QUIMICA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Buscar en Google
Añadir a Mi BVS
Imprimir
XML
PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de Semen / Motilidad Espermática / Espermatozoides / Superóxido Dismutasa / Criopreservación / Crioprotectores / Inulina / Malondialdehído Límite: Animals Idioma: En Revista: Cryo Letters Asunto de la revista: BIOLOGIA / QUIMICA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido