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PASTICCINO2 interacting with Golgi Anti-Apoptotic Proteins resists endoplasmic reticulum stress dependent on very long-chain fatty acids.
Tang, Xiao-Han; Zhou, Yan; He, Yu-Ting; Zhang, Wei; Chen, Xi; Tan, Jing; Guo, Kun; Liu, Yu-Ting; Zhao, Shu-Heng; Ning, Yi-Qiu; Sun, Yue; Li, Xiao-Fang.
Afiliación
  • Tang XH; School of Life Sciences, East China Normal University, 500 Dongchuan Rd., Shanghai, P R China.
  • Zhou Y; College of Forestry Engineering, Shandong Agriculture and Engineering University, 866 Nongganyuan Rd, Jinan, P R China.
  • He YT; School of Life Sciences, East China Normal University, 500 Dongchuan Rd., Shanghai, P R China.
  • Zhang W; School of Life Sciences, East China Normal University, 500 Dongchuan Rd., Shanghai, P R China.
  • Chen X; School of Life Sciences, East China Normal University, 500 Dongchuan Rd., Shanghai, P R China.
  • Tan J; School of Life Sciences, East China Normal University, 500 Dongchuan Rd., Shanghai, P R China.
  • Guo K; School of Life Sciences, East China Normal University, 500 Dongchuan Rd., Shanghai, P R China.
  • Liu YT; School of Life Sciences, East China Normal University, 500 Dongchuan Rd., Shanghai, P R China.
  • Zhao SH; School of Life Sciences, East China Normal University, 500 Dongchuan Rd., Shanghai, P R China.
  • Ning YQ; School of Life Sciences, East China Normal University, 500 Dongchuan Rd., Shanghai, P R China.
  • Sun Y; School of Life Sciences, East China Normal University, 500 Dongchuan Rd., Shanghai, P R China.
  • Li XF; School of Life Sciences, East China Normal University, 500 Dongchuan Rd., Shanghai, P R China.
J Exp Bot ; 2024 Aug 10.
Article en En | MEDLINE | ID: mdl-39126211
ABSTRACT
The endoplasmic reticulum (ER) is crucial for maintaining cell homeostasis because it is the primary site for synthesizing secreted and transmembrane proteins and lipids. The unfolded protein response (UPR) is activated to restore ER homeostasis under ER stress. However, the relationship between lipids and the ER stress response in plants is not well understood. Arabidopsis Golgi anti-apoptotic proteins (GAAPs) are involved in resisting ER stress. To elucidate the function of GAAPs, PASTICCINO2 (PAS2), involved in very long-chain fatty acid (VLCFA) synthesis, was found to interact with GAAPs and IRE1. Single pas2 and gaap1/gaap2pas2 double mutants exhibited increased seedling damage and impaired UPR response under chronic ER stress. Site mutation combined with genetic analysis revealed that the role of PAS2 in resisting ER stress depended on its VLCFA synthesis domain. VLCFA contents were upregulated under ER stress, which required GAAPs. Exogenous VLCFAs partially restored the defect in UPR upregulation caused by PAS2 or GAAP mutations under chronic ER stress. These findings demonstrate that the association of PAS2 with GAAPs confers plant resistance to ER stress by regulating VLCFA synthesis and the UPR. This provides a basis for further studies on the connection between lipids and cell fate decisions under stress.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Exp Bot Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Exp Bot Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido