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DArTseq-Based, High-Throughput Identification of Novel Molecular Markers for the Detection of Blackleg (Leptosphaeria Spp.) Resistance in Rapeseed.
Starosta, Ewa; Jamruszka, Tomasz; Szwarc, Justyna; Bocianowski, Jan; Jedryczka, Malgorzata; Grynia, Magdalena; Niemann, Janetta.
Afiliación
  • Starosta E; Department of Genetics and Plant Breeding, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland.
  • Jamruszka T; Department of Genetics and Plant Breeding, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland.
  • Szwarc J; Department of Genetics and Plant Breeding, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland.
  • Bocianowski J; Department of Mathematical and Statistical Methods, Poznan University of Life Sciences, Wojska Polskiego 28, 60-627 Poznan, Poland.
  • Jedryczka M; Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland.
  • Grynia M; IHAR Group, Borowo Department, Strzelce Plant Breeding Ltd., Borowo 35, 64-020 Czempin, Poland.
  • Niemann J; Department of Genetics and Plant Breeding, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland.
Int J Mol Sci ; 25(15)2024 Aug 01.
Article en En | MEDLINE | ID: mdl-39125985
ABSTRACT
Blackleg disease, caused by Leptosphaeria spp. fungi, is one of the most important diseases of Brassica napus, responsible for severe yield losses worldwide. Blackleg resistance is controlled by major R genes and minor quantitative trait loci (QTL). Due to the high adaptation ability of the pathogen, R-mediated resistance can be easily broken, while the resistance mediated via QTL is believed to be more durable. Thus, the identification of novel molecular markers linked to blackleg resistance for B. napus breeding programs is essential. In this study, 183 doubled haploid (DH) rapeseed lines were assessed in field conditions for resistance to Leptosphaeria spp. Subsequently, DArTseq-based Genome-Wide Association Study (GWAS) was performed to identify molecular markers linked to blackleg resistance. A total of 133,764 markers (96,121 SilicoDArT and 37,643 SNP) were obtained. Finally, nine SilicoDArT and six SNP molecular markers were associated with plant resistance to Leptosphaeria spp. at the highest significance level, p < 0.001. Importantly, eleven of these fifteen markers were found within ten genes located on chromosomes A06, A07, A08, C02, C03, C06 and C08. Given the immune-related functions of the orthologues of these genes in Arabidopsis thaliana, the identified markers hold great promise for application in rapeseed breeding programs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Polimorfismo de Nucleótido Simple / Brassica napus / Sitios de Carácter Cuantitativo / Estudio de Asociación del Genoma Completo / Resistencia a la Enfermedad / Leptosphaeria Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Polimorfismo de Nucleótido Simple / Brassica napus / Sitios de Carácter Cuantitativo / Estudio de Asociación del Genoma Completo / Resistencia a la Enfermedad / Leptosphaeria Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Suiza