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The Combination of Chitosan-Based Biomaterial and Cellular Therapy for Successful Treatment of Diabetic Foot-Pilot Study.
Humenik, Filip; Vdoviaková, Katarína; Kresáková, Lenka; Danko, Ján; Giretová, Mária; Medvecký, Lubomír; Lengyel, Peter; Babík, Ján.
Afiliación
  • Humenik F; Department of Morphological Sciences, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia.
  • Vdoviaková K; Department of Morphological Sciences, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia.
  • Kresáková L; Department of Morphological Sciences, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia.
  • Danko J; Department of Morphological Sciences, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia.
  • Giretová M; Division of Functional and Hybrid Systems, Institute of Materials Research of SAS, 040 01 Kosice, Slovakia.
  • Medvecký L; Division of Functional and Hybrid Systems, Institute of Materials Research of SAS, 040 01 Kosice, Slovakia.
  • Lengyel P; Clinic of Burns and Reconstructive Medicine, AGEL Hospital, 040 15 Kosice-Saca, Slovakia.
  • Babík J; Clinic of Burns and Reconstructive Medicine, AGEL Hospital, 040 15 Kosice-Saca, Slovakia.
Int J Mol Sci ; 25(15)2024 Aug 01.
Article en En | MEDLINE | ID: mdl-39125958
ABSTRACT
Diabetic foot ulceration is one of the most common complications in patients treated for diabetes mellitus. The presented pilot study describes the successful treatment of diabetic ulceration of the heel with ongoing osteomyelitis in a 39-year-old patient after using a combination of modified chitosan-based biomaterial in combination with autologous mesenchymal stem cells isolated from bone marrow and dermal fibroblasts. The isolated population of bone marrow mesenchymal stem cells fulfilled all of the attributes given by the International Society for Stem Cell Research, such as fibroblast-like morphology, the high expression of positive surface markers (CD29 99.1 ± 0.4%; CD44 99.8 ± 0.2% and CD90 98.0 ± 0.6%) and the ability to undergo multilineage differentiation. Likewise, the population of dermal fibroblasts showed high positivity for the widely accepted markers collagen I, collagen III and vimentin, which was confirmed by immunocytochemical staining. Moreover, we were able to describe newly formed blood vessels shown by angio CT and almost complete closure of the skin defect after 8 months of the treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Materiales Biocompatibles / Pie Diabético / Quitosano Límite: Adult / Humans / Male Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Eslovaquia Pais de publicación: Suiza
Texto completo
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Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Materiales Biocompatibles / Pie Diabético / Quitosano Límite: Adult / Humans / Male Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Eslovaquia Pais de publicación: Suiza