Transcriptomic Analysis of the Molecular Mechanism Potential of Grafting-Enhancing the Ability of Oriental Melon to Tolerate Low-Nitrogen Stress.

Zhu, Yulei; Sun, Ziqing; Wu, Hongxi; Cui, Caifeng; Meng, Sida; Xu, Chuanqiang

Zhu, Yulei; Sun, Ziqing; Wu, Hongxi; Cui, Caifeng; Meng, Sida; Xu, Chuanqiang.

Afiliación

Zhu Y; College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
Sun Z; Key Laboratory of Protected Horticulture (Ministry of Education), Shenyang Agricultural University, Shenyang 110866, China.
Wu H; Modern Protected Horticultural Engineering & Technology Center, Shenyang Agricultural University, Shenyang 110866, China.
Cui C; College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
Meng S; Key Laboratory of Protected Horticulture (Ministry of Education), Shenyang Agricultural University, Shenyang 110866, China.
Xu C; Modern Protected Horticultural Engineering & Technology Center, Shenyang Agricultural University, Shenyang 110866, China.

Int J Mol Sci ; 25(15)2024 Jul 27.

Article en En | MEDLINE | ID: mdl-39125797

ABSTRACT

ABSTRACT

Nitrogen is the primary nutrient for plants. Low nitrogen generally affects plant growth and fruit quality. Melon, as an economic crop, is highly dependent on nitrogen. However, the response mechanism of its self-rooted and grafted seedlings to low-nitrogen stress has not been reported previously. Therefore, in this study, we analyzed the transcriptional differences between self-rooted and grafted seedlings under low-nitrogen stress using fluorescence characterization and RNA-Seq analysis. It was shown that low-nitrogen stress significantly inhibited the fluorescence characteristics of melon self-rooted seedlings. Analysis of differentially expressed genes showed that the synthesis of genes related to hormone signaling, such as auxin and brassinolide, was delayed under low-nitrogen stress. Oxidative stress response, involved in carbon and nitrogen metabolism, and secondary metabolite-related differentially expressed genes (DEGs) were significantly down-regulated. It can be seen that low-nitrogen stress causes changes in many hormonal signals in plants, and grafting can alleviate the damage caused by low-nitrogen stress on plants, ameliorate the adverse effects of nitrogen stress on plants, and help them better cope with environmental stresses.

Asunto(s)

Cucurbitaceae; Perfilación de la Expresión Génica; Regulación de la Expresión Génica de las Plantas; Nitrógeno; Estrés Fisiológico; Transcriptoma; Nitrógeno/metabolismo; Estrés Fisiológico/genética; Cucurbitaceae/genética; Cucurbitaceae/crecimiento & desarrollo; Cucurbitaceae/metabolismo; Perfilación de la Expresión Génica/métodos; Plantones/genética; Plantones/crecimiento & desarrollo; Plantones/metabolismo; Proteínas de Plantas/genética; Proteínas de Plantas/metabolismo; Reguladores del Crecimiento de las Plantas/metabolismo

Palabras clave

RNA-seq; grafting; low-nitrogen stress; melon; squash

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Fisiológico / Regulación de la Expresión Génica de las Plantas / Cucurbitaceae / Perfilación de la Expresión Génica / Transcriptoma / Nitrógeno Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza

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