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Glycomacromolecules to Tailor Crowded and Heteromultivalent Glycocalyx Mimetics.
Blawitzki, Luca-Cesare; Bartels, Nina; Bonda, Lorand; Schmidt, Stephan; Monzel, Cornelia; Hartmann, Laura.
Afiliación
  • Blawitzki LC; Department for Organic Chemistry and Macromolecular Chemistry, Heinrich Heine University Duesseldorf, Universitätsstraße 1, 40225 Düsseldorf, Germany.
  • Bartels N; Department for Macromolecular Chemistry, University of Freiburg, Stefan-Meier-Str. 31, 79104 Freiburg i.Br., Germany.
  • Bonda L; Department for Experimental Medical Physics, Heinrich Heine University Duesseldorf, Universitätsstraße 1, 40225 Düsseldorf, Germany.
  • Schmidt S; Department for Organic Chemistry and Macromolecular Chemistry, Heinrich Heine University Duesseldorf, Universitätsstraße 1, 40225 Düsseldorf, Germany.
  • Monzel C; Department for Organic Chemistry and Macromolecular Chemistry, Heinrich Heine University Duesseldorf, Universitätsstraße 1, 40225 Düsseldorf, Germany.
  • Hartmann L; Department for Macromolecular Chemistry, University of Freiburg, Stefan-Meier-Str. 31, 79104 Freiburg i.Br., Germany.
Biomacromolecules ; 25(9): 5979-5994, 2024 Sep 09.
Article en En | MEDLINE | ID: mdl-39122664
ABSTRACT
The glycocalyx, a complex carbohydrate layer on cell surfaces, plays a crucial role in various biological processes. Understanding native glycocalyces' complexity is challenging due to their intricate and dynamic nature. Simplified mimics of native glycocalyces offer insights into glycocalyx functions but often lack molecular precision and fail to replicate key features of the natural analogues like molecular crowding and heteromultivalency. We introduce membrane-anchoring precision glycomacromolecules synthesized via solid-phase polymer synthesis (SPPoS) and thiol-induced, light-activated controlled radical polymerization (TIRP), enabling the construction of crowded and heteromultivalent glycocalyx mimetics with varying molecular weights and densities in giant unilamellar vesicles (GUVs). The incorporation and dynamics of glycomacromolecules in the GUVs are examined via microscopy and fluorescence correlation spectroscopy (FCS) and studies on lectin-carbohydrate-mediated adhesion of GUVs reveal inhibitory and promotional adhesion effects corresponding to different glycocalyx mimetic compositions, bridging the gap between synthetic models and native analogues.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicocálix Idioma: En Revista: Biomacromolecules Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicocálix Idioma: En Revista: Biomacromolecules Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos