Molecular structure and functional responses of IgM, IgT and IgD to Flavobacterium covae and Streptococcus iniae infection in Asian seabass (Lates calcarifer Bloch, 1790).

Uchuwittayakul, Anurak; Thangsunan, Pattanapong; Thangsunan, Patcharapong; Rodkhum, Channarong; Srisapoome, Prapansak

Uchuwittayakul, Anurak; Thangsunan, Pattanapong; Thangsunan, Patcharapong; Rodkhum, Channarong; Srisapoome, Prapansak.

Afiliación

Uchuwittayakul A; Laboratory of Aquatic Animal Health Management, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok, 10900, Thailand; Center of Excellence in Aquatic Animal Health Management (CE AAHM), Faculty of Fisheries, Kasetsart University, Bangkok, 10900, Thailand. Electronic addres
Thangsunan P; Division of Biochemistry and Biochemical Innovation, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand; Center of Excellence for Innovation in Chemistry, Research Laboratory on Advanced Materials for Sensor and Biosensor Innovation, Material Science Rese
Thangsunan P; Division of Biochemistry and Biochemical Innovation, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand; Office of Research Administration, Chiang Mai University, Chiang Mai, 50200, Thailand. Electronic address: tbscience@gmail.com.
Rodkhum C; Center of Excellence in Fish Infectious Diseases (CE FID), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand. Electronic address: channarong.r@chula.ac.th.
Srisapoome P; Laboratory of Aquatic Animal Health Management, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok, 10900, Thailand; Center of Excellence in Aquatic Animal Health Management (CE AAHM), Faculty of Fisheries, Kasetsart University, Bangkok, 10900, Thailand. Electronic addres

Fish Shellfish Immunol ; 153: 109823, 2024 Oct.

Article en En | MEDLINE | ID: mdl-39122096

ABSTRACT

ABSTRACT

The Asian seabass (Lates calcarifer) faces significant disease threats, which are exacerbated by intensive farming practices and environmental changes. Therefore, understanding its immune system is crucial. The current study presents a comprehensive analysis of immune-related genes in Asian seabass peripheral blood leukocytes (PBLs) using Iso-seq technology, identifying 16 key pathways associated with 7857 immune-related genes, comprising 634 unique immune-related genes. The research marks the first comprehensive report on the entire immunoglobulin repertoire in Asian seabass, revealing specific characteristics of immunoglobulin heavy chain constant region transcripts, including IgM (Cµ, ighm), IgT (Cτ, ight), and IgD (CÎ´, ighd). The study confirms the presence of membrane-bound form, ighmmb, ightmb, ighdmb of IgM, IgT and IgD and secreted form, ighmsc and ightsc of IgM and IgT, respectively, with similar structural patterns and conserved features in amino acids across immunoglobulin molecules, including cysteine residues crucial for structural integrity observed in other teleost species. In response to bacterial infections by Flavobacterium covae (formerly F. columnare genomovar II) and Streptococcus iniae, both secreted and membrane-bound forms of IgM (ighmmb and ighmsc) and IgT (ightmb and ightsc) show significant expression, indicating their roles in systemic and mucosal immunity. The expression of membrane-bound form IgD gene, ighdmb, predominantly exhibits targeted upregulation in PBLs, suggesting a regulatory role in B cell-mediated immunity. The findings underscore the dynamic and tissue-specific expression of immunoglobulin repertoires, ighmmb, ighmsc, ightmb, ightsc and ighdmb in Asian seabass, indicating a sophisticated immune response to bacterial pathogens. These findings have practical implications for fish aquaculture, and disease control strategies, serving as a valuable resource for advancing research in Asian seabass immunology.

Asunto(s)

Enfermedades de los Peces; Proteínas de Peces; Infecciones por Flavobacteriaceae; Flavobacterium; Inmunoglobulina D; Inmunoglobulina M; Inmunoglobulinas; Infecciones Estreptocócicas; Streptococcus iniae; Animales; Lubina/inmunología; Lubina/genética; Enfermedades de los Peces/inmunología; Proteínas de Peces/genética; Proteínas de Peces/inmunología; Proteínas de Peces/química; Infecciones por Flavobacteriaceae/inmunología; Infecciones por Flavobacteriaceae/veterinaria; Infecciones por Flavobacteriaceae/genética; Flavobacterium/fisiología; Inmunidad Innata/genética; Inmunoglobulina D/genética; Inmunoglobulina D/inmunología; Inmunoglobulina D/química; Inmunoglobulina M/inmunología; Inmunoglobulina M/genética; Inmunoglobulinas/genética; Inmunoglobulinas/inmunología; Infecciones Estreptocócicas/inmunología; Infecciones Estreptocócicas/veterinaria; Streptococcus iniae/fisiología

Palabras clave

Asian seabass; Flavobacterium covae; Full-length transcriptome; Immune-related genes; Immunoglobulins; Lates calcarifer; Streptococcus iniae

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Estreptocócicas / Inmunoglobulina D / Inmunoglobulina M / Inmunoglobulinas / Flavobacterium / Proteínas de Peces / Infecciones por Flavobacteriaceae / Enfermedades de los Peces / Streptococcus iniae Límite: Animals Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido

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