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Vascular tissues bioprinted with smooth muscle cell-only bioinks in support baths mimic features of native coronary arteries.
Figueroa-Milla, Andre E; DeMaria, William; Wells, Derrick; Jeon, Oju; Alsberg, Eben; Rolle, Marsha W.
Afiliación
  • Figueroa-Milla AE; Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA, United States of America.
  • DeMaria W; Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA, United States of America.
  • Wells D; Department of Biomedical Engineering, University of Illinois at Chicago, Chicago, IL, United States of America.
  • Jeon O; Department of Biomedical Engineering, University of Illinois at Chicago, Chicago, IL, United States of America.
  • Alsberg E; Department of Biomedical Engineering, University of Illinois at Chicago, Chicago, IL, United States of America.
  • Rolle MW; Departments of Mechanical & Industrial Engineering, Orthopaedic Surgery, and Pharmacology and Regenerative Medicine, University of Illinois at Chicago, Chicago, IL, United States of America.
Biofabrication ; 16(4)2024 Aug 29.
Article en En | MEDLINE | ID: mdl-39121893
ABSTRACT
This study explores the bioprinting of a smooth muscle cell-only bioink into ionically crosslinked oxidized methacrylated alginate (OMA) microgel baths to create self-supporting vascular tissues. The impact of OMA microgel support bath methacrylation degree and cell-only bioink dispensing parameters on tissue formation, remodeling, structure and strength was investigated. We hypothesized that reducing dispensing tip diameter from 27 G (210µm) to 30 G (159µm) for cell-only bioink dispensing would reduce tissue wall thickness and improve the consistency of tissue dimensions while maintaining cell viability. Printing with 30 G tips resulted in decreased mean wall thickness (318.6µm) without compromising mean cell viability (94.8%). Histological analysis of cell-only smooth muscle tissues cultured for 14 d in OMA support baths exhibited decreased wall thickness using 30 G dispensing tips, which correlated with increased collagen deposition and alignment. In addition, a TUNEL assay indicated a decrease in cell death in tissues printed with thinner (30 G) dispensing tips. Mechanical testing demonstrated that tissues printed with a 30 G dispensing tip exhibit an increase in ultimate tensile strength compared to those printed with a 27 G dispensing tip. Overall, these findings highlight the importance of precise control over bioprinting parameters to generate mechanically robust tissues when using cell-only bioinks dispensed and cultured within hydrogel support baths. The ability to control print dimensions using cell-only bioinks may enable bioprinting of more complex soft tissue geometries to generatein vitrotissue models.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vasos Coronarios / Ingeniería de Tejidos / Miocitos del Músculo Liso / Alginatos / Bioimpresión Límite: Animals Idioma: En Revista: Biofabrication Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vasos Coronarios / Ingeniería de Tejidos / Miocitos del Músculo Liso / Alginatos / Bioimpresión Límite: Animals Idioma: En Revista: Biofabrication Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido