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Mesoporous DNA-Co@C nanofibers knitted aptasensors performing onsite determination of trace kanamycin residues.
Yuan, Xueli; Kong, Jing; Xie, Ying; Liu, Xinxin; Zhang, Wei; Liu, Tao; Chu, Zhenyu; Jin, Wanqin.
Afiliación
  • Yuan X; State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing, 211816, China.
  • Kong J; State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing, 211816, China.
  • Xie Y; State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing, 211816, China.
  • Liu X; State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing, 211816, China.
  • Zhang W; Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, China.
  • Liu T; State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing, 211816, China. Electronic address: LT@njtech.edu.cn.
  • Chu Z; State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing, 211816, China. Electronic address: zychu@njtech.edu.cn.
  • Jin W; State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing, 211816, China.
Talanta ; 279: 126676, 2024 Nov 01.
Article en En | MEDLINE | ID: mdl-39121550
ABSTRACT
The abuse of kanamycin (KAN) poses an increasing threat to human health by contaminating agricultural and animal husbandry products, drinking water, and more. Therefore, the sensitive detection of trace KAN residues in real samples is crucial for monitoring agricultural pollution, ensuring food safety, and diagnosing diseases. However, traditional assay techniques for KAN rely on bulky instruments and complicated operations with unsatisfactory detection limits. Herein, we developed a novel label-free aptasensor to achieve ultrasensitive detection of KAN by constructing mesoporous DNA-cobalt@carbon nanofibers (DNA-Co@C-NFs) as the recognizer. Leveraging the extended π-conjugation structure, prominent surface area, and abundant pores, the Co@C-NFs can effectively load aptamer strands via π-π stacking interactions, serving as KAN capturer and reporter. Due to the change in DNA configuration upon binding KAN, this aptasensor presented an ultralow detection limit and ultra-wide linear range, along with favorable precision and selectivity. Using real tap water, milk, and human serum samples, the aptasensor accurately reported trace KAN levels. As a result, this convenient and rapid autosensing technique holds promise for onsite testing of other antibiotic residues in agriculture, food safety, and clinical diagnosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Carbono / Kanamicina / Técnicas Biosensibles / Cobalto / Aptámeros de Nucleótidos / Nanofibras Límite: Animals / Humans Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Carbono / Kanamicina / Técnicas Biosensibles / Cobalto / Aptámeros de Nucleótidos / Nanofibras Límite: Animals / Humans Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos