Elevated Salt or Angiotensin II Levels Induce CD38+ Innate Immune Cells in the Presence of Granulocyte-Macrophage Colony Stimulating Factor.

Smith, Hannah L; Goodlett, Bethany L; Navaneethabalakrishnan, Shobana; Mitchell, Brett M

Smith, Hannah L; Goodlett, Bethany L; Navaneethabalakrishnan, Shobana; Mitchell, Brett M.

Afiliación

Smith HL; Department of Medical Physiology, Texas A&M School of Medicine, Bryan, TX 77807, USA.
Goodlett BL; Department of Medical Physiology, Texas A&M School of Medicine, Bryan, TX 77807, USA.
Navaneethabalakrishnan S; Department of Medical Physiology, Texas A&M School of Medicine, Bryan, TX 77807, USA.
Mitchell BM; Department of Medical Physiology, Texas A&M School of Medicine, Bryan, TX 77807, USA.

Cells ; 13(15)2024 Aug 04.

Article en En | MEDLINE | ID: mdl-39120331

ABSTRACT

ABSTRACT

Hypertension (HTN) impacts almost half of adults, predisposing them to cardiovascular disease and renal damage. Salt-sensitive HTN (SSHTN) and angiotensin II (A2)-induced HTN (A2HTN) both involve immune system activation and renal innate immune cell infiltration. Subpopulations of activated [Cluster of differentiation 38 (CD38)] innate immune cells, such as macrophages and dendritic cells (DCs), play distinct roles in modulating renal function and blood pressure. It is unknown how these cells become CD38+ or which subtypes are pro-hypertensive. When bone marrow-derived monocytes (BMDMs) were grown in granulocyte-macrophage colony stimulating factor (GM-CSF) and treated with salt or A2, CD38+ macrophages and CD38+ DCs increased. The adoptive transfer of GM-CSF-primed BMDMs into mice with either SSHTN or A2HTN increased renal CD38+ macrophages and CD38+ DCs. Flow cytometry revealed increased renal M1 macrophages and type-2 conventional DCs (cDC2s), along with their CD38+ counterparts, in mice with either SSHTN or A2HTN. These results were replicable in vitro. Either salt or A2 treatment of GM-CSF-primed BMDMs significantly increased bone marrow-derived (BMD)-M1 macrophages, CD38+ BMD-M1 macrophages, BMD-cDC2s, and CD38+ BMD-cDC2s. Overall, these data suggest that GM-CSF is necessary for the salt or A2 induction of CD38+ innate immune cells, and that CD38 distinguishes pro-hypertensive immune cells. Further investigation of CD38+ M1 macrophages and CD38+ cDC2s could provide new therapeutic targets for both SSHTN and A2HTN.

Asunto(s)

Angiotensina II; Células Dendríticas; Factor Estimulante de Colonias de Granulocitos y Macrófagos; Inmunidad Innata; Macrófagos; Animales; Angiotensina II/farmacología; Factor Estimulante de Colonias de Granulocitos y Macrófagos/farmacología; Factor Estimulante de Colonias de Granulocitos y Macrófagos/metabolismo; Ratones; Inmunidad Innata/efectos de los fármacos; Macrófagos/efectos de los fármacos; Macrófagos/inmunología; Macrófagos/metabolismo; Células Dendríticas/efectos de los fármacos; Células Dendríticas/inmunología; Células Dendríticas/metabolismo; Hipertensión/inmunología; Ratones Endogámicos C57BL; ADP-Ribosil Ciclasa 1/metabolismo; Masculino; Monocitos/efectos de los fármacos; Monocitos/metabolismo; Monocitos/inmunología; Riñón/inmunología; Riñón/efectos de los fármacos

Palabras clave

CD38; dendritic cells; granulocyte-macrophage colony stimulating factor; hypertension; macrophages

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Dendríticas / Angiotensina II / Factor Estimulante de Colonias de Granulocitos y Macrófagos / Inmunidad Innata / Macrófagos Límite: Animals Idioma: En Revista: Cells Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google