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Mitochondrial NME6: A Paradigm Change within the NME/NDP Kinase Protein Family?
Proust, Bastien; Herak Bosnar, Maja; Cetkovic, Helena; Tokarska-Schlattner, Malgorzata; Schlattner, Uwe.
Afiliación
  • Proust B; Division of Molecular Medicine, Ruder Boskovic Institute, 10000 Zagreb, Croatia.
  • Herak Bosnar M; Division of Molecular Medicine, Ruder Boskovic Institute, 10000 Zagreb, Croatia.
  • Cetkovic H; Division of Molecular Biology, Ruder Boskovic Institute, 10000 Zagreb, Croatia.
  • Tokarska-Schlattner M; Univ. Grenoble Alpes, Inserm U1055, Lab. of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France.
  • Schlattner U; Univ. Grenoble Alpes, Inserm U1055, Lab. of Fundamental and Applied Bioenergetics (LBFA), 38058 Grenoble, France.
Cells ; 13(15)2024 Jul 30.
Article en En | MEDLINE | ID: mdl-39120309
ABSTRACT
Eukaryotic NMEs/NDP kinases are a family of 10 multifunctional proteins that occur in different cellular compartments and interact with various cellular components (proteins, membranes, and DNA). In contrast to the well-studied Group I NMEs (NME1-4), little is known about the more divergent Group II NMEs (NME5-9). Three recent publications now shed new light on NME6. First, NME6 is a third mitochondrial NME, largely localized in the matrix space, associated with the mitochondrial inner membrane. Second, while its monomeric form is inactive, NME6 gains NDP kinase activity through interaction with mitochondrial RCC1L. This challenges the current notion that mammalian NMEs require the formation of hexamers to become active. The formation of complexes between NME6 and RCC1L, likely heterodimers, seemingly obviates the necessity for hexamer formation, stabilizing a NDP kinase-competent conformation. Third, NME6 is involved in mitochondrial gene maintenance and expression by providing (d)NTPs for replication and transcription (in particular the pyrimidine nucleotides) and by a less characterized mechanism that supports mitoribosome function. This review offers an overview of NME evolution and structure and highlights the new insight into NME6. The new findings position NME6 as the most comprehensively studied protein in NME Group II and may even suggest it as a new paradigm for related family members.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mitocondrias Límite: Animals / Humans Idioma: En Revista: Cells Año: 2024 Tipo del documento: Article País de afiliación: Croacia Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mitocondrias Límite: Animals / Humans Idioma: En Revista: Cells Año: 2024 Tipo del documento: Article País de afiliación: Croacia Pais de publicación: Suiza