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Transposition with Tn3-family elements occurs through interaction with the host ß-sliding clamp processivity factor.
Tang, Yu; Zhang, Jianfeng; Guan, Jiahao; Liang, Wei; Petassi, Michael T; Zhang, Yumeng; Jiang, Xiaofei; Wang, Minggui; Wu, Wenjuan; Ou, Hong-Yu; Peters, Joseph E.
Afiliación
  • Tang Y; Department of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200123, China.
  • Zhang J; Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, China.
  • Guan J; State Key Laboratory of Microbial Metabolism, Joint International Laboratory on Metabolic & Developmental Sciences, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
  • Liang W; State Key Laboratory of Microbial Metabolism, Joint International Laboratory on Metabolic & Developmental Sciences, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
  • Petassi MT; Department of Laboratory Medicine, The First Affiliated Hospital of Ningbo University, Ningbo 315010, China.
  • Zhang Y; Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.
  • Jiang X; State Key Laboratory of Microbial Metabolism, Joint International Laboratory on Metabolic & Developmental Sciences, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
  • Wang M; Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China.
  • Wu W; Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, China.
  • Ou HY; Department of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200123, China.
  • Peters JE; State Key Laboratory of Microbial Metabolism, Joint International Laboratory on Metabolic & Developmental Sciences, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Nucleic Acids Res ; 52(17): 10416-10430, 2024 Sep 23.
Article en En | MEDLINE | ID: mdl-39119921
ABSTRACT
Tn3 family transposons are a widespread group of replicative transposons, notorious for contributing to the dissemination of antibiotic resistance, particularly the global prevalence of carbapenem resistance. The transposase (TnpA) of these elements catalyzes DNA breakage and rejoining reactions required for transposition. However, the molecular mechanism for target site selection with these elements remains unclear. Here, we identify a QLxxLR motif in N-terminal of Tn3 TnpAs and demonstrate that this motif allows interaction between TnpA of Tn3 family transposon Tn1721 and the host ß-sliding clamp (DnaN), the major processivity factor of the DNA replication machinery. The TnpA-DnaN interaction is essential for Tn1721 transposition. Our work unveils a mechanism whereby Tn3 family transposons can bias transposition into certain replisomes through an interaction with the host replication machinery. This study further expands the diversity of mobile elements that use interaction with the host replication machinery to bias integration.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Elementos Transponibles de ADN / Transposasas / Replicación del ADN Idioma: En Revista: Nucleic Acids Res Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Elementos Transponibles de ADN / Transposasas / Replicación del ADN Idioma: En Revista: Nucleic Acids Res Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido