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A structural peculiarity of Antarctic fish IgM drives the generation of an engineered mAb by CRISPR/Cas9.
Ametrano, Alessia; Miranda, Bruno; Moretta, Rosalba; Dardano, Principia; De Stefano, Luca; Oreste, Umberto; Coscia, Maria Rosaria.
Afiliación
  • Ametrano A; Institute of Biochemistry and Cell Biology, National Research Council of Italy, Naples, Italy.
  • Miranda B; Institute of Applied Sciences and Intelligent Systems, National Research Council of Italy, Naples, Italy.
  • Moretta R; Sanidrink s.r.l., Naples, Italy.
  • Dardano P; Institute of Applied Sciences and Intelligent Systems, National Research Council of Italy, Naples, Italy.
  • De Stefano L; Institute of Applied Sciences and Intelligent Systems, National Research Council of Italy, Naples, Italy.
  • Oreste U; Institute of Biochemistry and Cell Biology, National Research Council of Italy, Naples, Italy.
  • Coscia MR; Institute of Biochemistry and Cell Biology, National Research Council of Italy, Naples, Italy.
Front Bioeng Biotechnol ; 12: 1315633, 2024.
Article en En | MEDLINE | ID: mdl-39119272
ABSTRACT
IgM is the major circulating Ig isotype in teleost fish, showing in Antarctic fish unique features such as an extraordinary long hinge region, which plays a crucial role in antibody structure and function. In this work, we describe the replacement of the hinge region of a murine monoclonal antibody (mAb) with the peculiar hinge from Antarctic fish IgM. We use the CRISPR/Cas9 system as a powerful tool for generating the engineered mAb. Then, we assessed its functionality by using an innovative plasmonic substrate based on bimetallic nanoislands (AgAuNIs). The affinity constant of the modified mAb was 2.5-fold higher than that obtained from wild-type mAb against the specific antigen. Here, we show the suitability of the CRISPR/Cas9 method for modifying a precise region in immunoglobulin gene loci. The overall results could open a frontier in further structural modifications of mAbs for biomedical and diagnostic purposes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Bioeng Biotechnol Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Bioeng Biotechnol Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza