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Genomic characterization of carbapenemase-producing Enterobacterales from Dhaka food markets unveils the spread of high-risk antimicrobial-resistant clones and plasmids co-carrying bla NDM and mcr-1.1.
Nisa, Tania Tabassum; Sugawara, Yo; Hamaguchi, Shigeto; Takeuchi, Dan; Abe, Ryuichiro; Kuroda, Eisuke; Morita, Masatomo; Zuo, Hui; Ueda, Akiko; Nishi, Isao; Hossain, Nowrin; Hasan, Md Mahmudul; Siddiqee, Mahbubul H; Nakatani, Daisaku; Nakata, Ken; Akeda, Yukihiro.
Afiliación
  • Nisa TT; Global and Innovative Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan.
  • Sugawara Y; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
  • Hamaguchi S; Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
  • Takeuchi D; Division of Infection Control and Prevention, Osaka University Hospital, Osaka, Japan.
  • Abe R; Department of Transformative Analysis for Human Specimen, Graduate School of Medicine, Osaka University, Osaka, Japan.
  • Kuroda E; Division of Fostering Required Medical Human Resources, Center for Infectious Disease Education and Research (CiDER), Osaka University, Osaka, Japan.
  • Morita M; Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
  • Zuo H; Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
  • Ueda A; Division of Fostering Required Medical Human Resources, Center for Infectious Disease Education and Research (CiDER), Osaka University, Osaka, Japan.
  • Nishi I; Department of Transformative Infection Control Development Studies, Graduate School of Medicine, Osaka University, Osaka, Japan.
  • Hossain N; Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan.
  • Hasan MM; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
  • Siddiqee MH; Laboratory for Clinical Investigation, Osaka University Hospital, Osaka, Japan.
  • Nakatani D; Laboratory for Clinical Investigation, Osaka University Hospital, Osaka, Japan.
  • Nakata K; Molecular and Environmental Microbiology (MEM) Laboratory, Department of Mathematics and Natural Sciences (MNS), BRAC University, Dhaka, Bangladesh.
  • Akeda Y; Molecular and Environmental Microbiology (MEM) Laboratory, Department of Mathematics and Natural Sciences (MNS), BRAC University, Dhaka, Bangladesh.
JAC Antimicrob Resist ; 6(4): dlae124, 2024 Aug.
Article en En | MEDLINE | ID: mdl-39119043
ABSTRACT

Background:

The transmission of carbapenemase-producing Enterobacterales (CPE) in the external environment, especially through food, presents a significant public health risk.

Objectives:

To investigate the prevalence and genetic characteristics of CPE in food markets of Dhaka, Bangladesh, using WGS.

Methods:

CPE isolates were obtained from different food and water samples collected from food markets in the southern part of Dhaka, Bangladesh. The isolates subsequently underwent molecular typing, WGS employing both short- and long-read sequencers, and plasmid analysis.

Results:

This study unveiled an extensive spread of CPE, with no significant difference in contamination rates observed in samples (N = 136), including meat (n = 8), fish (n = 5), vegetables (n = 36) or various food-washed water (n = 65) from markets near hospitals or residential areas. Thirty-eight Enterobacterales from 33 samples carried carbapenemase genes (bla NDM-1, -4, -7, bla KPC-2, bla OXA-181 or bla IMI-1). Among these, the high-risk Escherichia coli ST410 clone was the most prevalent and distributed across various locations. Furthermore, the identification of IncHI2 plasmids co-harbouring resistance genes like bla NDM-5 and mcr-1.1, without discernible epidemiological connections, is a unique finding, suggesting their widespread dissemination.

Conclusions:

The analysis unveils a dynamic landscape of CPE dissemination in food markets, underscored by the proliferation of novel IncHI2 hybrid plasmids carrying both colistin- and carbapenem-resistance genes. This illuminates the ever-evolving landscape of antimicrobial resistance in Dhaka, urging us to confront its emergent challenges.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: JAC Antimicrob Resist Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: JAC Antimicrob Resist Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido