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Silymarin ameliorates diazinon-induced subacute nephrotoxicity in rats via the Keap1-Nrf2/heme oxygenase-1 signaling pathway.
Fath, Eman Mohamed; Bakery, Hatem H; El-Shawarby, Ragab M; Abosalem, Mohamed E S; Ibrahim, Samar S; Ebrahim, Nesrine; Hegazy, Ahmed Medhat.
Afiliación
  • Fath EM; Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, 13736, Qalyubia, Egypt.
  • Bakery HH; Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, 13736, Qalyubia, Egypt.
  • El-Shawarby RM; Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, 13736, Qalyubia, Egypt.
  • Abosalem MES; Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, 13736, Qalyubia, Egypt.
  • Ibrahim SS; Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, 13736, Qalyubia, Egypt.
  • Ebrahim N; Department of Histology and Cell Biology, Faculty of Medicine, Benha University, Benha, 13511, Egypt.
  • Hegazy AM; Stem Cell Unit, Faculty of Medicine, Benha University, Benha, 13511, Egypt.
Forensic Toxicol ; 2024 Aug 08.
Article en En | MEDLINE | ID: mdl-39117988
ABSTRACT

PURPOSE:

The goal of the current study was to clarify the potential molecular mechanism underlying the protective effects of silymarin (SIL) administration against diazinon-induced subacute nephrotoxicity, with a special emphasis on the role of the Kelch-like-associated protein-1 (Keap1)-nuclear factor erythroid 2-related factor 2 (Nrf2)-heme oxygenase-1 (HO-1) signaling pathway in minimizing the oxidative stress induced by diazinon (DZN).

METHODS:

Five equal groups of thirty adult male Wistar rats were created at random. Group 1 (G1) was maintained under typical control conditions and administered saline intragastrically (I/G) once daily for 4 weeks; G2 was administered olive oil I/G for 4 weeks; G3 was I/G administered silymarin daily for 4 weeks; G4 was I/G administered diazinon daily for 4 weeks. G5 was I/G administered silymarin daily 1 h before the I/G administration of the diazinon for 4 weeks. Blood samples were collected at the end of the experiment for the determination of complete blood cell count, and kidney function tests. Kidney specimens were collected for the evaluation of the oxidative markers, mRNA gene expression, protein markers, and histopathological examination.

RESULTS:

SIL reduced the renal dysfunction caused by DZN by restoring urea and creatinine levels, as well as oxidative indicators. Although the expression of Keap-1 was also elevated, overexpression of Nrf2 also enhanced the expression of HO-1, a crucial target enzyme of Nrf2.

CONCLUSIONS:

SIL is hypothesized to potentially aid in the prevention and management of nephrotoxicity caused by DZN.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Forensic Toxicol Año: 2024 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Japón
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Forensic Toxicol Año: 2024 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Japón